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History Of Genome Editing

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lecture 17
lydiaw4
Quiz by lydiaw4, updated more than 1 year ago
lydiaw4
Created by lydiaw4 about 10 years ago
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Resource summary

Question 1

Question
[blank_start]Miescher[blank_end] first isolated DNA. (He did it from white blood cells) This was in [blank_start]1869[blank_end].
Answer
  • Miescher
  • 1869

Question 2

Question
Endonucleases were used as a defense mechanism against [blank_start]viruses[blank_end]. The host genome was protected via [blank_start]methylation[blank_end].
Answer
  • viruses
  • methylation

Question 3

Question
Endonucleases are sequence specific, however, if there is one or two mismatches, they can still cut.
Answer
  • True
  • False

Question 4

Question
In the 1960s, [blank_start]Arber[blank_end] first identified restriction enzymes, but in 1970s, [blank_start]Smith[blank_end] was the first to isolate one. It was [blank_start]Hind II[blank_end].
Answer
  • Arber
  • Smith
  • Hind II

Question 5

Question
[blank_start]Gilbert[blank_end] purified the enzymatic activity of DNA ligase.
Answer
  • Gilbert

Question 6

Question
[blank_start]Cohen[blank_end] and [blank_start]Boyer[blank_end] first performed a bacterial transformation.
Answer
  • Cohen
  • Boyer

Question 7

Question
E. Coli replicate every
Answer
  • 20 minutes
  • 30 minutes
  • 45 minutes
  • hour

Question 8

Question
Trans[blank_start]formation[blank_end] is the term for bacteria given a transgene. Trans[blank_start]fection[blank_end] is when DNA is added to mammalian cells. Trans[blank_start]duction[blank_end] is viral DNA to bacteria.
Answer
  • formation
  • fection
  • duction

Question 9

Question
Northern blot is for [blank_start]RNA[blank_end]. Western is for [blank_start]protein[blank_end]. Southern is for [blank_start]DNA[blank_end].
Answer
  • RNA
  • protein
  • DNA

Question 10

Question
Which was the main method used to visualize DNA before PCR?
Answer
  • Southern blot
  • Northern blot
  • endonucleases

Question 11

Question
Which of these requires a radioactive isotope, P32?
Answer
  • Southern Blot
  • Northern Blot
  • Western Blot

Question 12

Question
Conventional cell culture began in the
Answer
  • 1940s
  • 1910s
  • 1960s
  • 1970s

Question 13

Question
[blank_start]Primary[blank_end] cell culture is established from tissue. Immortalized cell lines need [blank_start]telomerase[blank_end].
Answer
  • Primary
  • telomerase

Question 14

Question
The first transfection was done by [blank_start]Wigler[blank_end] in 1977. He introduced a part of [blank_start]herpes[blank_end] virus into a [blank_start]mouse[blank_end] cell line.
Answer
  • Wigler
  • herpes
  • mouse

Question 15

Question
The two types of genetic modification are [blank_start]transient[blank_end] (short term) or [blank_start]permanent[blank_end]
Answer
  • transient
  • permanent

Question 16

Question
Some methods of genetically modifying cells are [blank_start]electroporation[blank_end], l[blank_start]ipofectamin[blank_end], and [blank_start]viral[blank_end] vectors.
Answer
  • electroporation
  • ipofectamin
  • viral

Question 17

Question
[blank_start]Capecchi[blank_end] produced the first knock out mice.
Answer
  • Capecchi

Question 18

Question
Which 2 of these have the highest efficiency of HR?
Answer
  • ES cells
  • PGCs
  • Zygotes
  • Blastocysts

Question 19

Question
The technology used to generate the first transgenic animals was [blank_start]pronuclear injection[blank_end].
Answer
  • pronuclear injection

Question 20

Question
Pronuclear injection involves introducing a fragment of DNA into a [blank_start]male pronucleus[blank_end].
Answer
  • male pronucleus

Question 21

Question
Pronuclear injection can add a function, or downregulate a function.
Answer
  • True
  • False

Question 22

Question
Pronuclear injection is a common technique in livestock.
Answer
  • True
  • False

Question 23

Question
Which of these are reasons(that we discussed in class) why pronuclear injection is not typically used on livestock?
Answer
  • there is a lot of opposition to GMOs
  • long gestation
  • low efficiency (well under 20%)
  • expensive

Question 24

Question
When using lentivirus transduction on embryos, inject the virus into the
Answer
  • sub zona area
  • zona
  • media
  • zygote cell

Question 25

Question
SCNT done by skilled hands is usually successful.
Answer
  • True
  • False
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