Quiz
Environment Effect to Microbiology
Fragen in diesem Quiz (21)
1Who is the inventor of The Winogradsky Column?
- ASeiger Winogradsky
- BSergei Winogradsky
2The winogradsky column is as an artificial microbial ecosystem that serve a short term source of bacteria for isolating a pure culture.
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3Mud is supplemented with Calcium Sulphate as a buffer and Calcium Carbonate as a source of Carbon
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4Air is not prohibited in the Winogradsky column since the anaerobic microorganism could live below the surface where the Oxygen is not available. The column could be packed loosely but should be covered by different source of water.
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5Agar dilution tubes is a mixed culture diluted in molten agar for purifying anaerobic microorganisms.
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6General staining method includes all of these, except
- ADAPI (Diamido Phenylindole)
- BAO (Acridine Orange)
- CSYBR Green II
- DNone of the above
7DAPI, AO, and SYBR Green stain will be fluorescent under UV, but unable to be used to measure the quantity of microorganism in the sample.
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8Select every true statement below:
- ASergei Winogradsky first used the Winogradsky column to identify anaerobes and aerobes.
- BThe column was first used in 19th century to study soil microorganisms.
- CThe result of the column is shown by a change in color and the existence of colony patches in the soil. The existence of gas could also be detected by a crack in the soil.
- DA direct sunlight is necessary when storing the column.
9Select every true statement below:
- ASergei Winogradsky first used the Winogradsky column to identify anaerobes and aerobes.
- BThe column was first used in 19th century to study soil microorganisms.
- CThe result of the column is shown by a change in color and the existence of colony patches in the soil. The existence of gas could also be detected by a crack in the soil.
- DA direct sunlight is necessary when storing the column.
10In the Winogradsky Column, the up-most layer in the water column is oxic because algae and cyanobacteria produces O2 by photosynthesis. Decomposition process happens in the mud, leading to the production of organic acid, alcoholes, and H2 that provides a good environment for non-sulfate-reducing bacteria. These bacteria then produce H2S, triggering the growth of purple and green bacteria. These bacteria grow in the shape of layers and may bloom due to the lack of oxygen and/or oxygenic phototrophs.
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11Winogradsky columns have been used to enrich only the aerobic prokaryotes since the anaerobic ones are inhibited by anaerobic bacteria. The other purpose of using this columns is to test the hypothesis that an organism in the inoculum is able to degrade the specific compound that supplemented in the column. Using this column as a supply source of inocula for enrichment cultures is impossible.
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12Agar Dilution Tubes method uses a serial dilution in a molten agar to isolate a single colony of aerobic microorganism.
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13MPN (Most Probable Number) technique is used to estimate the number of microorganism in samples that have to be assessed regularly like food, wastewater, etc.
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14Choose the right statement below:
- ADAPI fluoresce blue and absorbs optimally at 450 nm
- BAO fluoresce orange or greenish orange and absorbs optimally at 400 nm
- CSYBR Green I fluoresce blue-green and absorbs optimally in 597 nm
- DNone of the above
- EThree of the options are right.
15Since DNA Staining such as DAPI and AO is highly specific, the viability of the cells could be assessed and measure the living cells. Thus, this technique is desirable to track species of microorganism in the environment.
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16The basis of differentiating between living and dead cells is whether or not the endoplasmic reticulum is intact. Dyes that fluoresce green and red are used, green for all bacteria, and red only stains dead bacteria. Viability staining like this could instantly give an information about the abundance and viability of microorganism observed.
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17The more specific fluorescent staining possible is by using fluorescent antibodies. This antibody provides a great information about the microorganism observed. However, this technique is rather expensive and time-consuming.
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18The downside of using the Fluorescent Antibodies to stain are
- AA pure culture is often optional.
- BPreparation of specific antibodies against the microorganism is obligatory.
- CPreparation of specific antibodies is prohibited.
- DA pure culture is obligatory.
- ENone of the above.
19gfp gene is able to be used on both oxic and anoxic environment because the need of O2 is optional for GFP protein to fluoresce. That is the reason why gfp method is widely used in many fields to track microorganism and its life cycle.
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20A reporter gene is a gene that able to report an activity of translation.
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21Why does general fluorescent staining method can't provide a sufficient information about the diversity of organisms in nature?
- AThe range of size of the organisms is very wide.
- Bit's not possible to determine live and dead cells.
- CThe phylogenetic diversity of microorganisms is not revealed.
Informationen
- Beschreibung
- College Environmental Microbiology Quiz am Environment Effect to Microbiology, erstellt von raifedora am 21/01/2014.
- Autor
- raifedora
- Sprache
- English
- Aufrufe
- 81
- Schlagwörter
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