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CC2 MU2U2
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- 1What is the EC Code for ALP? What does ALP catalyze?EC 3.1.3.1
ALP catalyzes the hydrolysis of various phosphomonoesters at an alkaline pH. It is to liberate inorganic phosphate from organic ones with the production of alcohol. - 2TRUE or FALSE: ALP is a SPECIFIC enzyme capable of reacting with only one substrateFALSE: ALP is a non-specific enzyme capable of reacting with many different substrates
- 3ALP is an enzyme involved during ____ in the intestine and calcification process in the bone through ______LIPID TRANSPORT; HYDROLYSIS of ORTHOPHOSPHATE
- 4Phosphate absorption is facilitated by what isoenzymes of ALPBoth intestinal and kidney
- 5In the liver, ALP is mainly found in? How about in the bone?Sinusoidal and bile canalicular membrane; osteoblasts involved in the production of bone matrix
- 6Cite the origins of ALP IsoformsChromosome 1: KLB
Chromosome 2: PI - 7What are the lectins used to retard the migration of bone ALP?Germ wheat lectin and neuraminidase with the exception that neuraminidase does not retard intestinal ALP
- 8In the reversible reaction of lactate dehydrogenase, it strongly favors the reduction of what?Reduction of pyruvate to lactate
- 9What are the LD isoenzymes?LD1 (H4)
LD2 (H3M)
LD3 (H2M2)
LD4 (HM3)
LD5 (M4)
LDx or c (4 x or c): present in post-pubertal testes
LD6- commonly observed in sera of patients with arteriosclerosis cardiovascular failure - 10What is an LD flip? state the hours at which the LD 1 rise, peak and normalizeLD flip is when the serum concentration of LD 1 is higher or greater than that of LD 2. This usually happens following AMI however it is not a marker for AMI as it is not specific to cardiac tissue
12-24 hrs, peak 48-72 hrs, remain elevated within 10 days - 11Highest elevations of LD occurs in?Hemolytic anemia, megaloblastic anemia, pernicious anemia
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