Microscope

Preparation of a slide for compound light microscope

Fixation

Dehydration

Embedded in paraffin wax and cut it in to estremely thin slices by a microtome.

Cleared an mounted in a trasnparent glass

Immersed in colorful stains and dyes to differentiate the components

Microcospe slides preparation

Place a drop of fluid in the center of the slide.

Position sample on liquid, using tweezers.

At an angle, place one side of the cover slip against the slide making contact with outer edge of the liquid drop.

Lower the cover slowly, avoiding air bubbles.

Remove excess water with the paper towel.

Freeze drying

FREEZE - The product is completely frozen, usually in a vial, flask or tray.

VACUUM - The product is then placed under a deep vacuum, well below the triple point of water.

DRY – Heat energy is then added to the product causing the ice to sublime.