Introduce el texto aquí

Introduce el texto aquí

1. PTMScan Proteomics form Cell Signaling Techonology

   • Combines antibody-based enrichment with mass spec to both indentify and quantify post tranlationally modified peptides
     • Key factor: ANTIBODIES USED FOR THE ENRICHMENT
       • Tipycally a part of a protein or a full one is used when a antibody is developed
         • The final product is nice and specific for one given protein site
       • The antibodies used for PGM scan are degenerate peptides libraries
         • The products are hugely specific for a targeted motif
         • Flexible process
           • Can be developed using cell lines, tissues, plant material, whole organisms, plasma, sorted cells
           • 1. Starts taking the material and digestive peptides, peptides are not at same level than proteins complexes
             • 2. Taken peptides are enriched with one or more of specialized PTM motif antibodies
               • 3. Run mass spec is ran to obtain peptide identifications.
                 • 4. Quantification and data analysis are maden
           • PTM TYPES OF PRODUCTS AND SERVICES
             • 1. Phosphorylation: Tyrosine, serine or threonine
               • Enrichment can be made using anitbodies or metal ions on beads
                 • According to how you enrich your antibody and the metal affinity
                   • You get complimentary pools of phospho peptides identified
                     • Good coverage of the tyrosine phosphorylation
               • 2. Ubiquitination
                 • Degenerate peptide library approach is used to make an antibody against the dye glicine remanent
                 • 3. Acetylation
                   • 4. Methylation on lysine and arginine
                     • 5. Caspase Cleavage
                       • 6. Acylation
                         • Lisine
                           • Succinylation, malonylation
                         • 7. Signaling Pathways
                           • Uses multiple cocktails of the site specific antibodies
                             • Specific signaling pathways or protein types like: kinase
                             • Doesn´t have enrichment step
                           • 8. KinomeView
                             • Used whenthe type of motif antibody to be used is not clear with PTM
                             • 9. Pathway Analysis
                               • Protein-protein interaction network analysis
                               • Studios in "de novo" generated pathways