MICROBIAL METABOLISM

MICROBIAL METABOLISM

1. METABOLISM

   • CATABOLISM
     • Enzyme-regulated chemical reactions that release energy by breaking down complex organic molecules to simpler form.
   • ANABOLISM
     • Formation of larger molecules from smaller molecules and requires energy

2. ENZYME

   • CLASSIFICATION
     • Oxidoreductase
     • Transferase
     • Hydrolase
     • Lyases
     • Isomerase
     • Ligase
   • GENERAL PROPERTIES
     • Posses active site
     • Catalytic efficiency is faster than uncatalyzed reactions
     • Reactions are specific
     • Require carrier molecules
       • Co-enzymes
       • Cofactors
     • Enzyme activity can be regulated to suit cells specific needs
   • FACTORS AFFECTING ENZYMATIC REACTIONS
     • Substrate, product concentration
     • pH
     • Temperature
     • Enzyme inhibitors
   • MICHAELIS MENTEN KINETICS
     • Enzyme activity is dependent on substrate concentration
   • TYPES OF INHIBITION
     • Competitive inhibition
       • Due to similar shape of substrate and inhibitor. Inhibitor binds at the active site.
     • Non-competitive inhibition
       • Interact with allosteric site. Binding causes shape changes of active site
     • Feedback inhibition
       • The product will inhibit enzymatic reaction

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