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What is this test for? List media, purpose(s), indicator, molecule, as well as incubation temp & time, as well as procedure
What is this test for? List media, purpose(s), indicator, molecule, as well as incubation temp & time, as well as procedure
What Bacteria used for H2S & Motility?
What is this pathway?
Enterobacter aerogenes, what is this also known as?
Lab 9: IMViC Series:
All enterics initially produce _____ from ______.
Lab 9 IMViC series:
Pyruvic acid further metabolized by which 2 pathways?
final electron acceptor in aerobic respiration?
final electron acceptor in anaerobic respiration?
final electron acceptor fermentation?
what are two parts to gelatin slant?
what bacteria used for lactose fermentation?
Was a durham tube used for lactose fermentation?
Which test had these purposes:
ferment specific sugars (glu, lac, suc)
produce gas (CO2)
produce hydrogen sulfide (H2S) by reducing sulfur
Which test had this purpose:
to determine if microbes reduce sulfur-containing compounds to sulfides during the process of metabolism & to determine if a microbe is motile
Which test had this purpose:
To determine if a microbe ferments the carbohydrate lactose and to determine if they produce gas as a by-product of carbohydrate fermentation
Which test used these bacteria?
Escherichia coli
Proteus vulgaris
Staphylococcus aureus
Which test used these control org.?
Escherichia coli
Alcaligenes faecalis
Salmonella typhimurium
Staphylococcus aureus
Shigella flexneri
Proteus vulgaris
What control organisms used for Lipid Hydrolysis?
What test purpose is this:
To determine whether a microbe can hydrolyze a lipid (fat) to fatty acid and glycerol, which can be further reduced and used as a source of ATP
What test used Spirit Blue Agar (SBA) media plates?
What was the enzyme and indicator for Lipid Hydrolysis?
What was incubation for lipid hydrolysis?
What is this and what are the results?
What control organisms used for Gelatin Hydrolysis?
What is this result for TSI test say?
What is this result for TSI test say?
What does this TSI result say?
Tubes showing black precipitate in TSI tubes mean what?
Tube displaying displaced/broken agar means what?
_________?for gas by-product = no air bubble in Durham tube
(Lactose Fermentation Test)
____________?for carbohydrate utilization with acidic end product = yellow color
(Lactose Fermentation Test)
_______?for carbohydrate utilization with acidic end product = yellow color
(lactose fermentation test)
________? gas by-product = air bubble in Durham tube
(lactose fermentation test)
___________?for carbohydrate utilization but growth was present as shown by peptone use to produce alkaline end product = dark pink/fuchsia color
(lactose fermentation test)
______________?seen by neutral end products = original orange/red color (same as negative control)
(Lactose fermentation test)
Is this negative or positive for lactose fermentation?
Why is this no growth in lactose fermentation test?
what reaction pathway is this?
TSI results for Alcaligenes faecalis?
What test is this?
What does IMViC stand for?
What is IMViC tests mainly used for?
Normal intestinal flora
Opportunistic pathogens such as
Full names for short G (-) non-spore forming rod-shaped bact. in intestinal tract?
S. typhimurium
S. flexneri
What test purpose is this:
To determine if a microbe can split indole from the amino acid tryptophan
What test is this:
Media = 1% tryptone broth (Not SIM agar like your lab manual says)•
Enzyme = Tryptophanase
•Indicator = Kovac’s reagent
What test is this:
selects for organisms found in the family, Enterobacteriaceae, and differentiates between members of that family that can produce tryptophanase & those that cannot
What test and what are results?
What was positive result in Methyl Red test? What was negative?
What were the two bacterial species used in IMViC tests?
What did use to inoculate in indole test?
Which test?
Is positive/negative?
What bacteria?
Which test?
Is positive/negative?
What bacteria?
Describe Urease test purpose, media, enzyme, and indicator
Organisms used in Urease Test?
How long to incoluate Urease?
Describe Nitrate reduction test purpose, media, enzyme, and indicator:
What organisms used for nitrate test?
What test is this?:
Purpose: to determine if microbes can break down hydrogen peroxide (H2O2) into H2O and O2
Media = Tryptic Soy Agar plates
Enzyme = catalase
Indicator = H2O2
What organisms used in Catalase Test?
What test is this?:
Purpose: to determine if a microbe uses the electron transport chain as the final phase in aerobic respiration
Media = Tryptic Soy Agar plates
Enzyme = cytochrome oxidase
Indicator = p-aminodimethylaniline oxalate (Oxidase reagent)
Control Organisms for Oxidase Test?
Did Urease, Nitrate, Catalase, and Oxidase test all need 37 degrees C for 24-48 hours incubation?
What test? What results- which bacteria?
