21083050
Description
Flashcards by Emma Ragan, updated more than 1 year ago
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Created by Emma Ragan
about 4 years ago
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| Question | Answer |
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What is this test for? List media, purpose(s), indicator, molecule, as well as incubation temp & time, as well as procedure
Image:
H2 S3 (binary/octet-stream)
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Test: H2S & motility -Positive for H2S if black precip. -Positive for motility if turbidity is seen -Media: sim agar -Molecule: sodium thiosulfate -Indicator: ferrous sulfate -Differ. betwn motile/nonmotile bact. as well as H2S & non-H2S producing bact. - 37 degrees C, 24-48 hours -Stab really straight w/ needle aseptic |
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What is this test for? List media, purpose(s), indicator, molecule, as well as incubation temp & time, as well as procedure
Image:
H2 S4 (binary/octet-stream)
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-Positive for motility if turbidity is seen -Media: sim agar -Molecule: sodium thiosulfate -Indicator: ferrous sulfate -Differ. betwn motile/nonmotile bact. as well as H2S & non-H2S producing bact. - 37 degrees C, 24-48 hours -Stab really straight w/ needle aseptic |
| What Bacteria used for H2S & Motility? | Escherichia coli Proteus vulgaris Staphylococcus aureus |
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What is this pathway?
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H2 S (binary/octet-stream)
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H2S production via pathway 1, please memorize for practical |
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Image:
H2 S2 (binary/octet-stream)
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H2S production via pathway 2, please memorize for practical |
| Enterobacter aerogenes, what is this also known as? | Also known as Klebsiella aerogenes |
| Lab 9: IMViC Series: All enterics initially produce _____ from ______. | produce pyruvic acid from glucose metabolism |
| Lab 9 IMViC series: Pyruvic acid further metabolized by which 2 pathways? | 1. mixed acid pathway 2. butylene glycol pathway |
| final electron acceptor in aerobic respiration? | O2 |
| final electron acceptor in anaerobic respiration? | inorganic molecule such as NO3- or SO 2-/4 |
| final electron acceptor fermentation? | an organic molecule that is carbon containing |
| what are two parts to gelatin slant? | slant & butt |
| what bacteria used for lactose fermentation? | Escherichia coli G(-) rod Micrococcus luteus G(+) cocci Staphylococcus aureus G(+) cocci |
| Was a durham tube used for lactose fermentation? | yes |
| Which test had these purposes: ferment specific sugars (glu, lac, suc) produce gas (CO2) produce hydrogen sulfide (H2S) by reducing sulfur | Triple Sugar Iron test |
| Which test had this purpose: to determine if microbes reduce sulfur-containing compounds to sulfides during the process of metabolism & to determine if a microbe is motile | sim agar deep |
| Which test had this purpose: To determine if a microbe ferments the carbohydrate lactose and to determine if they produce gas as a by-product of carbohydrate fermentation | Pr-lactose fermentation |
| Which test used these bacteria? Escherichia coli Proteus vulgaris Staphylococcus aureus | H2S and Motility Test |
| Which test used these control org.? Escherichia coli Alcaligenes faecalis Salmonella typhimurium Staphylococcus aureus Shigella flexneri Proteus vulgaris | Triple Sugar Iron Test (TSI) |
| What control organisms used for Lipid Hydrolysis? | Staphylococcus aureus G(+) cocci Escherichia coli G(-) rod |
| What test purpose is this: To determine whether a microbe can hydrolyze a lipid (fat) to fatty acid and glycerol, which can be further reduced and used as a source of ATP | Lipid Hydrolysis |
| What test used Spirit Blue Agar (SBA) media plates? | Lipid Hydrolysis |
| What was the enzyme and indicator for Lipid Hydrolysis? | Enzyme= lipase Indicator=olive oil |
| What was incubation for lipid hydrolysis? | 37 degrees C for 24 hours |
| What is this and what are the results? | Lipid hydrolysis on SBA plate; left shows positive (S. aur) zone of clearing for enzyme lipase production, right shows negative (E. coli) zone of clearing |
| What control organisms used for Gelatin Hydrolysis? | Bacillus cereus G(+) rod Escherichia coli G(-) rod Micrococcus luteus G(+) cocci Staphylococcus aureus G(+) cocci |
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What is this result for TSI test say?
Image:
Tsi3 (binary/octet-stream)
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yellow slant/butt = fermentation of lactose and/or sucrose |
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What is this result for TSI test say?
Image:
Tsi (binary/octet-stream)
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red slant / red butt = no fermentation |
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What does this TSI result say?
Image:
Tsi2 (binary/octet-stream)
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red slant/yellow butt= glucose fermentation |
| Tubes showing black precipitate in TSI tubes mean what? | H2S production |
| Tube displaying displaced/broken agar means what? | CO2 production |
| _________?for gas by-product = no air bubble in Durham tube (Lactose Fermentation Test) | Negative |
| ____________?for carbohydrate utilization with acidic end product = yellow color (Lactose Fermentation Test) | positive |
| _______?for carbohydrate utilization with acidic end product = yellow color (lactose fermentation test) | Positive |
| ________? gas by-product = air bubble in Durham tube (lactose fermentation test) | positive |
| ___________?for carbohydrate utilization but growth was present as shown by peptone use to produce alkaline end product = dark pink/fuchsia color (lactose fermentation test) | Negative |
| ______________?seen by neutral end products = original orange/red color (same as negative control) (Lactose fermentation test) | No growth |
| Is this negative or positive for lactose fermentation? | Negative for carbohydrate utilization but growth was present as shown by peptone use to produce alkaline end product = dark pink/fuchsia color |
| Why is this no growth in lactose fermentation test? | No Growth seen by neutral end products = original orange/red color (same as negative control) |
| what reaction pathway is this? | Lipid Hydrolysis |
| TSI results for Alcaligenes faecalis? | red slant, orange butt, no fermentation or gas production |
| What test is this? | Gelatin Hydrolysis: 3 types of results as well: -positive for rapid liquefaction (after 2 day incubation) -positive for slow liquefaction (after 7 day incubation) -negative for liquefaction (no liquefaction in agar deeps after 7 days incub.) |
| What does IMViC stand for? | Indole Test Methyl Red Test Voges-Proskauer test i (nothing) Citrate Test |
| What is IMViC tests mainly used for? | Mainly used to identify bacteria found in the family, Enterobacteriaceae •Short, G(-), non-spore forming, rod-shaped bacteria that can be found in the intestinal tract |
| Normal intestinal flora | Escherichia coli & Enterobacter aerogenes |
| Opportunistic pathogens such as | Proteus vulgaris, & Klebsiella pneumoniae |
| Full names for short G (-) non-spore forming rod-shaped bact. in intestinal tract? S. typhimurium S. flexneri | Salmonella typhimurium Shigella flexneri |
| What test purpose is this: To determine if a microbe can split indole from the amino acid tryptophan | Indole test |
| What test is this: Media = 1% tryptone broth (Not SIM agar like your lab manual says)• Enzyme = Tryptophanase •Indicator = Kovac’s reagent | Indole Test |
| What test is this: selects for organisms found in the family, Enterobacteriaceae, and differentiates between members of that family that can produce tryptophanase & those that cannot | Indole Test |
| What test and what are results? | Indole Test - Positive: pink top after Kovac reagent (E. coli) -Negative: yellow top after Kovac's reagent (E. aero) |
| What was positive result in Methyl Red test? What was negative? | -positive E. coli -negative E. aero |
| What were the two bacterial species used in IMViC tests? | Escherichia coli Enterobacter aerogenes |
| What did use to inoculate in indole test? | loop |
| Which test? Is positive/negative? What bacteria? | Citrate Test Positive Enterobacter aerogenes (bromothymol blue indicator) |
| Which test? Is positive/negative? What bacteria? | Citrate Test Negative Esherichia coli (no growth/color change) |
| Describe Urease test purpose, media, enzyme, and indicator | Purpose: To determine if a microbe can hydrolyze urea to ammonia (NH3) and carbon dioxide (CO2) Media = Urea broth Enzyme = urease Indicator = phenol red |
| Organisms used in Urease Test? | Control organisms: Escherichia coli Proteus mirabilis |
| How long to incoluate Urease? | 37°C for 24-48 hours |
| Describe Nitrate reduction test purpose, media, enzyme, and indicator: | Purpose: to determine if microbes can reduce nitrate (NO3-) to nitrite (NO2-) or even further to nitric oxide (NO), nitrous oxide (N2O), ammonia (NH3), or nitrogen (N2) Media = Nitrate broth (Potassium nitrate: KNO3) Enzyme = nitrate reductase and/or nitrite reductase Indicator = sulfanilic acid (Solution A), α-naphthylamine (Solution B), & zinc dust |
| What organisms used for nitrate test? | Control organisms: Alcaligenes faecalis Escherichia coli Pseudomonas aeruginosa |
| What test is this?: Purpose: to determine if microbes can break down hydrogen peroxide (H2O2) into H2O and O2 Media = Tryptic Soy Agar plates Enzyme = catalase Indicator = H2O2 | Catalase Test |
| What organisms used in Catalase Test? | Control organisms: Enterococcus faecalis Escherichia coli |
| What test is this?: Purpose: to determine if a microbe uses the electron transport chain as the final phase in aerobic respiration Media = Tryptic Soy Agar plates Enzyme = cytochrome oxidase Indicator = p-aminodimethylaniline oxalate (Oxidase reagent) | Oxidase Test |
| Control Organisms for Oxidase Test? | Control organisms: Klebsiella pneumoniae Pseudomonas aeruginosa |
| Did Urease, Nitrate, Catalase, and Oxidase test all need 37 degrees C for 24-48 hours incubation? | Yes |
| What test? What results- which bacteria? | Mannitol Salt Agar Test -Positive mannitol ferm.= yellow (S. aureus) (S. epi) -Negative mannitol ferm.= pink/red (S. sapro) |
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