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lecture 17

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History Of Genome Editing

Question 1 of 25

1

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first isolated DNA. (He did it from white blood cells) This was in .

Explanation

Question 2 of 25

1

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Endonucleases were used as a defense mechanism against . The host genome was protected via .

Explanation

Question 3 of 25

1

Endonucleases are sequence specific, however, if there is one or two mismatches, they can still cut.

Select one of the following:

  • True
  • False

Explanation

Question 4 of 25

1

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In the 1960s, first identified restriction enzymes, but in 1970s, was the first to isolate one. It was .

Explanation

Question 5 of 25

1

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purified the enzymatic activity of DNA ligase.

Explanation

Question 6 of 25

1

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and first performed a bacterial transformation.

Explanation

Question 7 of 25

1

E. Coli replicate every

Select one of the following:

  • 20 minutes

  • 30 minutes

  • 45 minutes

  • hour

Explanation

Question 8 of 25

1

Fill the blank spaces to complete the text.

Trans is the term for bacteria given a transgene. Trans is when DNA is added to mammalian cells. Trans is viral DNA to bacteria.

Explanation

Question 9 of 25

1

Northern blot is for . Western is for . Southern is for .

Drag and drop to complete the text.

    RNA
    protein
    DNA

Explanation

Question 10 of 25

1

Which was the main method used to visualize DNA before PCR?

Select one of the following:

  • Southern blot

  • Northern blot

  • endonucleases

Explanation

Question 11 of 25

1

Which of these requires a radioactive isotope, P32?

Select one of the following:

  • Southern Blot

  • Northern Blot

  • Western Blot

Explanation

Question 12 of 25

1

Conventional cell culture began in the

Select one of the following:

  • 1940s

  • 1910s

  • 1960s

  • 1970s

Explanation

Question 13 of 25

1

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cell culture is established from tissue. Immortalized cell lines need .

Explanation

Question 14 of 25

1

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The first transfection was done by in 1977. He introduced a part of virus into a cell line.

Explanation

Question 15 of 25

1

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The two types of genetic modification are (short term) or

Explanation

Question 16 of 25

1

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Some methods of genetically modifying cells are , l, and vectors.

Explanation

Question 17 of 25

1

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produced the first knock out mice.

Explanation

Question 18 of 25

1

Which 2 of these have the highest efficiency of HR?

Select one or more of the following:

  • ES cells

  • PGCs

  • Zygotes

  • Blastocysts

Explanation

Question 19 of 25

1

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The technology used to generate the first transgenic animals was .

Explanation

Question 20 of 25

1

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Pronuclear injection involves introducing a fragment of DNA into a .

Explanation

Question 21 of 25

1

Pronuclear injection can add a function, or downregulate a function.

Select one of the following:

  • True
  • False

Explanation

Question 22 of 25

1

Pronuclear injection is a common technique in livestock.

Select one of the following:

  • True
  • False

Explanation

Question 23 of 25

1

Which of these are reasons(that we discussed in class) why pronuclear injection is not typically used on livestock?

Select one or more of the following:

  • there is a lot of opposition to GMOs

  • long gestation

  • low efficiency (well under 20%)

  • expensive

Explanation

Question 24 of 25

1

When using lentivirus transduction on embryos, inject the virus into the

Select one of the following:

  • sub zona area

  • zona

  • media

  • zygote cell

Explanation

Question 25 of 25

1

SCNT done by skilled hands is usually successful.

Select one of the following:

  • True
  • False

Explanation

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