Which of the following statements are true about chromatography? Select all that apply.
It separates mixtures into its distinct constituents.
It consists of stationary and mobile phases.
The solvent used is water-based.
Chromatography can only separate molecules based on their size.
TLC stands for .
For which of the following biological molecules would you use water as the mobile phase? Check all that apply.
Label this diagram:
The rate at which different molecules move along the stationary phase depends on...
Which of the following statements best explains how different molecules 'climb' chromatography paper at different rates?
Polar molecules travel faster because they don't form hydrogen bonds with the paper.
Polar molecules travel faster because they form hydrogen bonds with the paper.
Non-polar molecules travel faster because they don't form hydrogen bonds with the paper.
Non-polar molecules travel faster because they form hydrogen bonds with the paper.
Once a chromatography run has completed, you can identify the components in the mixture by finding their Rf values.
Rf = x / y
Which statements are true about this formula?
x is the distance travelled by the solvent
x is the distance travelled by the component
y is the distance travelled by the solvent
y is the distance travelled by the component
Three ways of visualising the molecular components separated during chromatography include shining a UV light
different coloured lights
infrared light( a UV light, white light, different coloured lights, infrared light ) over a TLC plate
gas chromatography column
mobile phase( TLC plate, gas chromatography column, mobile phase ), spraying ninhydrin
amino acid molecules
sodium hydrogen carbonate( ninhydrin, amino acid molecules, starch, sodium hydrogen carbonate ) on amino acid molecules
non-reducing sugars( amino acid molecules, triglyceride molecules, reducing sugars, non-reducing sugars ), or adding iodine crystals
copper sulphate( iodine crystals, chlorine gas, alkaline solution, copper sulphate ).