Chromatography

Daniel Brand
Quiz by Daniel Brand, updated more than 1 year ago
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Biology (Biological Molecules) Quiz on Chromatography, created by Daniel Brand on 02/20/2017.

Resource summary

Question 1

Question
Which of the following statements are true about chromatography? Select all that apply.
Answer
  • It separates mixtures into its distinct constituents.
  • It consists of stationary and mobile phases.
  • The solvent used is water-based.
  • Chromatography can only separate molecules based on their size.

Question 2

Question
TLC stands for [blank_start]thin[blank_end] [blank_start]layer[blank_end] [blank_start]chromatography[blank_end].
Answer
  • thin
  • layer
  • chromatography

Question 3

Question
For which of the following biological molecules would you use water as the mobile phase? Check all that apply.
Answer
  • Amino acids
  • Fats
  • Glucose
  • Oils

Question 4

Question
Label this diagram:
Answer
  • Beaker
  • Stationary phase
  • Mobile phase
  • Watch glass
  • Chromatography paper
  • Solvent
  • Spot of mixture
  • Beaker
  • Pencil line
  • Watch glass
  • Stationary phase
  • Beaker
  • Pencil line
  • Solvent
  • Solvent
  • Stationary phase
  • Spot of mixture
  • Chromatography paper
  • Watch glass
  • Solvent
  • Pencil line
  • Beaker
  • Chromatography paper
  • Spot of mixture
  • Spot of mixture
  • Chromatography paper
  • Beaker
  • Watch glass
  • Stationary phase

Question 5

Question
The rate at which different molecules move along the stationary phase depends on...
Answer
  • Size
  • Polarity
  • Solubility
  • Electronic charge

Question 6

Question
Which of the following statements best explains how different molecules 'climb' chromatography paper at different rates?
Answer
  • Polar molecules travel faster because they don't form hydrogen bonds with the paper.
  • Polar molecules travel faster because they form hydrogen bonds with the paper.
  • Non-polar molecules travel faster because they don't form hydrogen bonds with the paper.
  • Non-polar molecules travel faster because they form hydrogen bonds with the paper.

Question 7

Question
Once a chromatography run has completed, you can identify the components in the mixture by finding their Rf values. Rf = x / y Which statements are true about this formula?
Answer
  • x is the distance travelled by the solvent
  • x is the distance travelled by the component
  • y is the distance travelled by the solvent
  • y is the distance travelled by the component

Question 8

Question
Three ways of visualising the molecular components separated during chromatography include shining [blank_start]a UV light[blank_end] over a [blank_start]TLC plate[blank_end], spraying [blank_start]ninhydrin[blank_end] on [blank_start]amino acid molecules[blank_end], or adding [blank_start]iodine crystals[blank_end].
Answer
  • a UV light
  • white light
  • different coloured lights
  • infrared light
  • TLC plate
  • gas chromatography column
  • mobile phase
  • ninhydrin
  • amino acid molecules
  • starch
  • sodium hydrogen carbonate
  • amino acid molecules
  • triglyceride molecules
  • reducing sugars
  • non-reducing sugars
  • iodine crystals
  • chlorine gas
  • alkaline solution
  • copper sulphate
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