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21259927
Carotenos 2º método
Description
carotenos
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carotenos
grado superior
Flowchart by
Jorge Revilla
, updated more than 1 year ago
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Created by
Jorge Revilla
about 4 years ago
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Resource summary
Flowchart nodes
Inicio
5 mg de mustra liofilizada
1mL MeOH/Cl3CH/ tris-HCl (1:2:1)
Extraemos
Añadimos alfa-tocoferol como patrón interno
Centrifugar y recuper la fase orgánica
Speed vac
Redisolver en 100 uL de acetato de etilo
Analizar empleando UPLC-PDA
Fin
La longitud de onda es 285nm
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