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Quiz on Application of Molecular Biology to Medicine , created by MPusey on 05/01/2015.

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Application of Molecular Biology to Medicine

Question 1 of 25

1

What type of chromosome does G-banding "line-up"?

Select one of the following:

  • Metaphase

  • Anaphase

  • Telophase

  • Prophase

Explanation

Question 2 of 25

1

What stain does G-banding use?

Select one of the following:

  • Giemsa

  • Chrome red

  • Methyl-blue

  • Green

Explanation

Question 3 of 25

1

G-banding can only detect large abnormalities. True or false?

Select one of the following:

  • True
  • False

Explanation

Question 4 of 25

1

What does FISH stand for?

Select one of the following:

  • Fluorescent in situ hybridization

  • Fluorescent in site hydrolysis

  • Familial in situ hybridization

  • Familial in situ hydrolysis

Explanation

Question 5 of 25

1

You don't need to know what abnormality you are looking for when using FISH. True or false?

Select one of the following:

  • True
  • False

Explanation

Question 6 of 25

1

Why do you need to know what abnormality you are looking for when using FISH?

Select one of the following:

  • Because it uses fluorescent probes to bind to specific sequences

  • Because it used restriction enzymes marked with fluorescent probes to cut DNA at a specific sequence

Explanation

Question 7 of 25

1

How can you tell if there is an abnormality using the FISH technique?

Select one of the following:

  • Because some of the markers will not light up as they have not been able to anneal to bits of DNA sequence as they are missing

  • Because all of the markers will light up because there are specific sequences of DNA that cause genetic disorders

  • Because if there were no abnormalities the markers would not show up at all

Explanation

Question 8 of 25

1

FISH can show slightly smaller abnormalities than G-banding, but they still have to fairly large. True or false?

Select one of the following:

  • True
  • False

Explanation

Question 9 of 25

1

What does QF-PCR stand for?

Select one of the following:

  • Quantitative fluorescence Polymerase Chain Reaction

  • Qualitative fluorescence polymerase chain reaction

  • Quantitative familial polymerase chain reaction

  • Qualitative familial polymerase chain reaction

Explanation

Question 10 of 25

1

What is QF-PCR used to detect?

Select one of the following:

  • Trisomy syndromes

  • Abnormalities in chromosome size

  • Deletion syndromes

  • Abnormalities in chromosome banding

Explanation

Question 11 of 25

1

What is the name for trisomy 13?

Select one of the following:

  • Pattau's syndrome

  • Edward's syndrome

  • Down's syndrome

Explanation

Question 12 of 25

1

What is the name for trisomy 18?

Select one of the following:

  • Pattau's syndrome

  • Edward's syndrome

  • Down's syndrome

Explanation

Question 13 of 25

1

What is the name for trisomy 21?

Select one of the following:

  • Pattau's syndrome

  • Edward's syndrome

  • Down's syndrome

Explanation

Question 14 of 25

1

Which biological technique is quickest?

Select one of the following:

  • FISH

  • G-banding

  • QF-PCR

Explanation

Question 15 of 25

1

You have to know what abnormality you are looking for when using QF-PCR. True or false?

Select one of the following:

  • True
  • False

Explanation

Question 16 of 25

1

What sort of abnormalities does array-CGH detect?

Select one of the following:

  • Larger abnormalities

  • Smaller abnormalities

Explanation

Question 17 of 25

1

What is DNA labelled with in array-CGH?

Select one of the following:

  • Fluorescent dye

  • Coloured markers

  • Radiation

Explanation

Question 18 of 25

1

What does the CGH in array-CGH stand for?

Select one of the following:

  • Comparative genomic hybridization

  • Comparative genomic hydrolysis

  • Comparative genetic hybridization

  • Comparative genetic hydrolysis

Explanation

Question 19 of 25

1

How can you tell if there is an abnormality in the DNA when using array-CGH?

Select one of the following:

  • The relative fluorescence of the sample and control strand will be different

  • The relative radiation of the sample and control strand will be different

  • The length of the sample and control strand will be different when run through gel electrophoresis

Explanation

Question 20 of 25

1

Which two molecular biological techniques look at the actual base sequence when looking for genetic abnormalities?

Select one of the following:

  • G-banding

  • FISH

  • QF-PCR

  • Array-CGH

  • Sanger sequencing

  • Next generation sequencing

Explanation

Question 21 of 25

1

Which of the following is cheaper and less time consuming?

Select one of the following:

  • Sanger sequencing

  • Next generation sequencing

Explanation

Question 22 of 25

1

Why do we only sequence exons when using sanger sequencing?

Select one of the following:

  • Because the introns do not code for proteins so are not important to us

  • Because it is time consuming and expensive

  • Because introns rarely have mutations that cause genetic diseases

  • Because we are unable to isolate the introns to sequence them

Explanation

Question 23 of 25

1

What machine is used in sanger sequencing to read the sequence of bases?

Select one of the following:

  • An electropheragram

  • A Geiger counter

  • A microscope

Explanation

Question 24 of 25

1

Not all changes to the base sequence cause disease. True or false?

Select one of the following:

  • True
  • False

Explanation

Question 25 of 25

1

Why is it better to use next generation sequencing when you don't know what you are looking for?

Select one of the following:

  • Because it is easy to sequence a whole genome

  • Because sanger sequencing would take too long

  • Because next generation sequencing is more accurate

  • Because next generation sequencing is less expensive

Explanation

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