Week 8: Translocation & fusion genes

what is the difference between intrachromosomal and inter chromosomal abberations?

What is the difference between a balanced and an unbalanced translocation?

Which 3 steps are required for gene fusions?

Name 3 reasons for gene fusions

What are 3 consequences of oncogenic chromosomal translocations

Which 3 methods can be used to detect translocations and fusions?

Can FISH be done on FFPE or only of FF material

What is the difference between a break apart FISH and a fusion FISH

What chromosomal abberations can be seen with FISH?

Why would you used RNA seq instead of DNA seq (NGS/ Sanger)?

For translocation detection: When do you use RNA seq, and when FISH

How does Archer RNA seq work?

Name 2 pros and 2 cons of Archer

What is Nanostring used for?

Name 2 pros and 2 cons of Nanostring

Name 2 pros and 2 cons of RNA sequencing

What markers are used for the detection of B and T cells in IHC?

BCR rearrangement involves combinations of different fragments. Which fragments are combined in the light and which in the heavy chain

On which region of the BCR gene do primers bind to detect clonality for B cell malignancies?

What are the pitfalls of gene rearrangement analysis using TCR/BCR?

What is the difference between lymphomas and leukemia?

What 3 molecular things are testen in lymphomas