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Lecture quizzes

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A compilation of all of the quizzes from lecture on relevant topics for the final. I forgot to write down the quizzes for topics 25 and 26 though so those are missing.
skhal
Quiz by skhal, updated more than 1 year ago
skhal
Created by skhal over 9 years ago
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Resource summary

Question 1

Question
Which property is not essential for the origin of life?
Answer
  • The need to store information
  • The need to duplicate information
  • The need to change information
  • The need to make proteins
  • The need to convert into favorable reactions

Question 2

Question
Why is it thought that ribozymes are likely to be a legacy from an ancestral RNA world?
Answer
  • They are more stable than RNAs
  • When they act, they require the presence of protein cofactors
  • They act without the participation of protein cofactors.
  • They can be made spontaneously without a DNA template.
  • They are made by proteins

Question 3

Question
What was probably the first carrier of genetic information in primitive organisms?
Answer
  • DNA that could self-replicate
  • Proteins
  • Polypeptides
  • RNA that could self-replicate
  • Lipids

Question 4

Question
________ can be used to purify transcription activator proteins
Answer
  • Gel mobility shift assays
  • DNA affinity chromatography
  • Centrifugation
  • DNA footprinting
  • ChIP assays

Question 5

Question
Sequence specific DNA binding proteins frequently have a region that binds in the major groove because:
Answer
  • It is smaller
  • It contains sequence specific information
  • The minor groove is not available
  • It is positively charged
  • It is negatively charged

Question 6

Question
The following secondary structures are known to specifically interact with DNA (select all that apply):
Answer
  • Alpha helix
  • Beta sheet
  • Unstructured loops
  • Double helix

Question 7

Question
What happens at the lac operon in the absence of glucose and presence of lactose?
Answer
  • No expression
  • Low expression
  • The lac repressor binds the promoter
  • High expression
  • The CAP protein doesn't bind to the promoter

Question 8

Question
Multiple binding sites for a repressor is advantageous because:
Answer
  • There are too many repressors in cells
  • DNA must be bent to allow transcription
  • Repressors are only active when bound to many sites at once
  • This increases the effective concentration of the repressor
  • Repressors need multiple binding sites to bind ligands

Question 9

Question
An operator is:
Answer
  • A protein
  • A protein binding site
  • The same as a promoter
  • A type of repressor
  • The start site for RNA synthesis

Question 10

Question
Mammalian x-chromosome inactivation:
Answer
  • Occurs only in humans
  • Prevents females from being mosaics
  • Is not necessary
  • Is necessary for dosage compensation
  • Only occurs in males

Question 11

Question
CpG islands are generally found in
Answer
  • Inactive genes
  • Introns
  • Exons
  • Promoters of constantly active genes
  • Promoters of rarely expressed genes

Question 12

Question
Deamination of methyl-cytosine:
Answer
  • Does not occur
  • Changes methyl-cytosine to thymine
  • Only occurs in the sequence CG
  • Results in genomic imprinting
  • Occurs only in expressed genes

Question 13

Question
Sex determination in drosophila is due to:
Answer
  • Riboswitches
  • Alternative splicing
  • Alternative 3' end cleavage
  • Localization of mRNA
  • Alternative translational initiation

Question 14

Question
Mirco RNAs (miRNAs):
Answer
  • Are the same as rRNAs
  • Are not encoded by the genome
  • Are found only in bacteria
  • Are involved in gene repression
  • Are part of ribosomes

Question 15

Question
Attenuation involves regulation of:
Answer
  • Translation
  • Replication
  • Recombination
  • Transcription
  • Proteolysis

Question 16

Question
Ion exchange chromatography separates:
Answer
  • Proteins, based on size
  • Proteins, based on surface charge
  • Proteins, based on source

Question 17

Question
FRET is used to study:
Answer
  • Protein structure
  • Protein molecular weight
  • Protein/protein interaction
  • Protein sequence
  • Protein denaturation

Question 18

Question
SDS is used in protein gels to (select all that apply)...
Answer
  • Make glassware clean up easier
  • Help denature proteins
  • To create a uniform mass/charge ratio
  • To reduce disulfide bonds

Question 19

Question
DNA libraries are used to bank (select all that apply):
Answer
  • Genomic DNA
  • RNA
  • cDNA
  • Artificial chromosomes

Question 20

Question
Dideoxy nucleotides are used in DNA sequences to:
Answer
  • Denature the DNA
  • Hybridize the DNA
  • Chain terminate the DNA
  • Elongate the DNA
  • Transcribe the DNA

Question 21

Question
Hybridization of nucleic acids refers to:
Answer
  • A renaturation technique
  • An electrophoretic technique
  • A cloning technique
  • A forensic technique
  • A purification technique
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