457002
Descripción
Test por gina_evans0312, actualizado hace más de 1 año
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Creado por gina_evans0312
hace más de 10 años
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Pregunta 1
Pregunta
Name the two methods of physical fragmentation
Respuesta
-
Shearing- filtering through a fine needed
-
Sonication
-
Acidification
-
Boiling
Pregunta 2
Pregunta
Non-random shearing involves the use of helicases
Respuesta
- True
- False
Pregunta 3
Pregunta
Which of the following cuts most frequently?
Respuesta
-
4 cutters
-
6 cutters
-
8 cutters
Pregunta 4
Pregunta
A 4 cutter cuts a 4 bp sequence, and so on
Respuesta
- True
- False
Pregunta 5
Pregunta
The staggered ends of two different enzymes can be ligated together, but afterwards cannot be cut by the same enzymes
Respuesta
- True
- False
Pregunta 6
Pregunta
When dealing with DNA-cutting enzymes, what does the word degenerate mean?
Respuesta
-
The ends they produce don't last long
-
They can cut more than one sequence
-
Where in the sequence they cut changes
Pregunta 7
Pregunta
What conditions are necessary for a partial digest?
Respuesta
-
Not enough time
-
Less enzyme
-
Wrong enzyme conditions
-
A four cutter
Pregunta 8
Pregunta
Why could a partial digest be done?
Respuesta
-
To get longer fragments and more likely preserve the gene
-
To save time
-
To prevent the DNA from degrading
Pregunta 9
Pregunta
Why is E.Coli most often used as a cloning vector?
Respuesta
-
It has a rapid generation time
-
Its DNA is easy to manipulate
-
Its DNA is easy to get hold of
-
They don't mutate much
Pregunta 10
Pregunta
Bacteriophages can be used to transplant vectors into E.Coli
Respuesta
- True
- False
Pregunta 11
Pregunta
How does M13 infect the male E.Coli?
Respuesta
-
Binds to pilus surface
-
Enters through bacterial pores
-
Direct injection of DNA
Pregunta 12
Pregunta
Once inside the cell, the ssDONA of M13...
Respuesta
-
Is convered to dsDNA that acts like a plasmid
-
Is integrated into the bacterial chromosome
-
Is cut up by restriction enzymes ans integrated into the bacterial chromosome
Pregunta 13
Pregunta
TEVEN and Lambda enter the bacteria directly through the pore
Respuesta
- True
- False
Pregunta 14
Pregunta
What is the point of the resistance genes in this Generation 1 plasmid
Image:
Gen_1_Plasmid (image/jpg)
Respuesta
-
Tetracycline is interrupted- E.Coli with no resistance have had the recombinant plasmid inserted
-
The recombinant DNA is inserted at the EcrR1 site
-
E.Coli with ampicillin resistance have taken up a version of the plasmid
Pregunta 15
Respuesta
-
The small subunit of LacZ
-
The large subunit of LacZ
-
Ampicillin
-
Tetracycline
Pregunta 16
Pregunta
Once the LacZ subunit has been assembled, what form does LacZ take?
Image:
Lac_Z (image/png)
Respuesta
-
Monomer
-
Dimer
-
Tetramer
-
Hexamer
Pregunta 17
Pregunta
For Gen 2 plasmid testing to work, the LacZ gene must be removed from the E.Coli, and a plasmid for the large LacZ subunit added
Respuesta
- True
- False
Pregunta 18
Pregunta
Why is the dish impregnated with X-Gal?
Respuesta
-
It goes from blue to white in the presence of LacZ
-
It goes from white to blue in the presence of LacZ
-
It goes from white to blue in the presence of the small subunit of LacZ
Pregunta 19
Pregunta
If the gene is successfully inserted, the colony will remain white
Respuesta
- True
- False
Pregunta 20
Pregunta
A Phagemid is virally inserted DNA in plasmid form
Respuesta
- True
- False
Pregunta 21
Pregunta
Why would a vector be given an M13 origin and inserted with M13?
Respuesta
-
The M13 phage will treat the plasmid as its own DNA and replicate it for secretion
-
The phage will insert the vector into the chromosome
-
The phage will prevent the bacteria from destroying the plasmid
Pregunta 22
Pregunta
Cosmids are plasmids with...?
Respuesta
-
Cos lambda sites
-
Cos beta sites
-
Cos gamma sites
Pregunta 23
Pregunta
Which of the following has the largest limit?
Respuesta
-
BAC's
-
YAC's
-
MAC's
Pregunta 24
Pregunta
Why do you remove a phosphate from the ends of bacterial DNA using a phosphatase?
Image:
Remove_P (image/png)
Respuesta
-
To prevent it self ligating
-
To prevent it degrading
-
To prevent it getting destroyed
Pregunta 25
Pregunta
Why mix enzyme T4 and ATP together with the DNA to be inserted?
Respuesta
-
Adding AMP to ligated ends gives easily ligable high energy form
-
ATP & T4 remove the phosphates from the ends of the DNA to prevent self annealing
-
T4 can only ligate DNA in the presence of ATP
Pregunta 26
Pregunta
A vector must be used because E.Coli cannot take up linear DNA
Respuesta
- True
- False
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