Test
DNA Fragmentation and Cloning Vectors
Preguntas del test (26)
1Name the two methods of physical fragmentation
- AShearing- filtering through a fine needed
- BSonication
- CAcidification
- DBoiling
2Non-random shearing involves the use of helicases
- AVerdadero
- BFalso
3Which of the following cuts most frequently?
- A4 cutters
- B6 cutters
- C8 cutters
4A 4 cutter cuts a 4 bp sequence, and so on
- AVerdadero
- BFalso
5The staggered ends of two different enzymes can be ligated together, but afterwards cannot be cut by the same enzymes
- AVerdadero
- BFalso
6When dealing with DNA-cutting enzymes, what does the word degenerate mean?
- AThe ends they produce don't last long
- BThey can cut more than one sequence
- CWhere in the sequence they cut changes
7What conditions are necessary for a partial digest?
- ANot enough time
- BLess enzyme
- CWrong enzyme conditions
- DA four cutter
8Why could a partial digest be done?
- ATo get longer fragments and more likely preserve the gene
- BTo save time
- CTo prevent the DNA from degrading
9Why is E.Coli most often used as a cloning vector?
- AIt has a rapid generation time
- BIts DNA is easy to manipulate
- CIts DNA is easy to get hold of
- DThey don't mutate much
10Bacteriophages can be used to transplant vectors into E.Coli
- AVerdadero
- BFalso
11How does M13 infect the male E.Coli?
- ABinds to pilus surface
- BEnters through bacterial pores
- CDirect injection of DNA
12Once inside the cell, the ssDONA of M13...
- AIs convered to dsDNA that acts like a plasmid
- BIs integrated into the bacterial chromosome
- CIs cut up by restriction enzymes ans integrated into the bacterial chromosome
13TEVEN and Lambda enter the bacteria directly through the pore
- AVerdadero
- BFalso
14What is the point of the resistance genes in this Generation 1 plasmid
- ATetracycline is interrupted- E.Coli with no resistance have had the recombinant plasmid inserted
- BThe recombinant DNA is inserted at the EcrR1 site
- CE.Coli with ampicillin resistance have taken up a version of the plasmid
15What gene is disrupted in Gen 2 plasmids?
- AThe small subunit of LacZ
- BThe large subunit of LacZ
- CAmpicillin
- DTetracycline
16Once the LacZ subunit has been assembled, what form does LacZ take?
- AMonomer
- BDimer
- CTetramer
- DHexamer
17For Gen 2 plasmid testing to work, the LacZ gene must be removed from the E.Coli, and a plasmid for the large LacZ subunit added
- AVerdadero
- BFalso
18Why is the dish impregnated with X-Gal?
- AIt goes from blue to white in the presence of LacZ
- BIt goes from white to blue in the presence of LacZ
- CIt goes from white to blue in the presence of the small subunit of LacZ
19If the gene is successfully inserted, the colony will remain white
- AVerdadero
- BFalso
20A Phagemid is virally inserted DNA in plasmid form
- AVerdadero
- BFalso
21Why would a vector be given an M13 origin and inserted with M13?
- AThe M13 phage will treat the plasmid as its own DNA and replicate it for secretion
- BThe phage will insert the vector into the chromosome
- CThe phage will prevent the bacteria from destroying the plasmid
22Cosmids are plasmids with...?
- ACos lambda sites
- BCos beta sites
- CCos gamma sites
23Which of the following has the largest limit?
- ABAC's
- BYAC's
- CMAC's
24Why do you remove a phosphate from the ends of bacterial DNA using a phosphatase?
- ATo prevent it self ligating
- BTo prevent it degrading
- CTo prevent it getting destroyed
25Why mix enzyme T4 and ATP together with the DNA to be inserted?
- AAdding AMP to ligated ends gives easily ligable high energy form
- BATP & T4 remove the phosphates from the ends of the DNA to prevent self annealing
- CT4 can only ligate DNA in the presence of ATP
26A vector must be used because E.Coli cannot take up linear DNA
- AVerdadero
- BFalso
Información
- Descripción
- Year 2 Test sobre DNA Fragmentation and Cloning Vectors, creado por gina_evans0312 el 29/12/2013.
- Autor
- gina_evans0312
- Idioma
- English
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- 602
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