Fichas
Cell Signalling VI
Fichas del conjunto (22)
- 1Functions of apoptosisEmbryogenesis - Sculpting limbs/removal of redundant structures
Maintain Cell # - 2What is PCDElimination of cells damaged beyond repair
- 3What kinda damageDNA Damage
Repair mech. can't cope w/ the damage
Misfloded protein accumulation - 4Morphological features/ ultrastructure changesCell shrinkage
Chromatin condensation
IC content fragging + Membrane blebbing
Formation of AB - 5Caspases (4)1. Cys at AS
2. Cleave aspartic acid residues of target proteins.
3. Synthesised as inactive proteins.
4. Undergo proteolytic autocleavage of aspartic acid residues to become active. - 6Chain reaction (5)1. Autocleavage
2. Activate effector caspases
3. Cleave nuclear lamin
4. Effector + other caspases
5. Cleave cytosolic proteins. - 7Hallmark cleavage of Chr. DNACleave protein that prevents endonuclease activity.
DNA endonuclease cut into internucleosomal units of 180-200 bp. - 8Testing for apoptosisDNA laddering in electrophoresis
- 9What is the key "engulf me" signal?Phosphotidylserine
- 10MOAUsually found inner side of mem.
Move to outer side during apoptosis (caspase mediated).
Phagocytes like macrophages/neutrophils recognise PTS or other apoptopic signals of AB. - 11Intrinsic molecular pathway of Apoptosis (Mito)1. Lack of trophic factor-inducing signal i.e. GF/Survival F withdrawal.
2. DNA damage via P53 (Chemo)
3. Protein misfolding (E.R. stress). - 12What is intrinsic pathway + dependent on?Release of Cytochrome C from mitochondria
- 13Which molecules promote apoptosis?Pro
BAD
BAX
BAK - 14Which molecules inhibit apoptosis?BCL-2
BCL-XL
Block the action of BAX/BAK - 15How do BAK/BAX work?Form channels in out mito mem. to release Cytochrome C
- 16Signalling pathway1. Apoptosis stimulus
2. BAK/BAX +
3. Cytochrome + adaptor protein APAF-1
4. Assembly of adaptor//CCC
5. Recruitment of procaspase 9
6. Formation of apoptosome
7. + of procaspase 9 within apoptosome
8. Caspase cascade leading to apoptosis - 17GF/SF inhibition of apoptosisIncrease transcription/translation of anti-apoptopic molecules like BCL-2
Stimulation of PKB which Pi and - BAD - 18Extrinsic Pathway initiationReceptor mediated
Binding of TNF to their R
Kills cells targeted by the immune system - 19Cellular targetsCancer cells
Virus-infected cells
Excess lymphocytes at end of immune response - 20Key features of Death receptorsEC domain
Transmembrane domain
IC domain containing the Death Domains - 21Key features of AdaptorDeath Domains interact w/ DD of receptors
Death Effector Domains which interact w/ DED domains of initiator procaspases. - 22MOA1. Trimeric ligand causes R trimerisation
2. Two cytosolic adaptor proteins FADD + TRADD and procaspase-8 are recruited by the +TNF
3. The 2 procaspase-8 molecules + each other to initiate caspase cascade.
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- El usuario ha eliminado su información de asignaturas Fichas sobre Cell Signalling VI, creado por Usuario eliminado el 02/04/2018.
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- Creado por J yadonknow · 2 abr 2018
- Copiado por J yadonknow · 2 abr 2018
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