Fichas
Cell Signalling II
Fichas del conjunto (18)
- 12' Messengers + signal transductioncAMP
PKA
cGMP
PKG - 2Ligand-induced production of cAMPProduced from ATP by effector enzyme adenylyl cyclase
- 3Adenylyl Cyclase2 transmem domains joined by a cat IC domain
ATP degraded to 5'MP by a cAMP phosphodiesterase - 4cAMP mediatorPKA
- 5Guanylyl Cyclase functionConverts GTP into diffusable cGMP
- 62 formsMembrane bound - + by external L
Soluble cytoplasmic form* -
+ by intrinsic mol. e.g. NO - 7How is cGMP degradedDegraded by cGMP phosphodioesterase
- 8PIP2 +PiPIP2 -> PIP3 via PL3K
PIP3*->PDK1->PKB-> Cell proliferation - 9PIP3*->PIP2Via PTEN - a tumour surpressor
- 10Breakdown of PIP2 in LBLPLC splits PIP2
DAG-> PKC
InsP3-> Ca2+ release - 11How does Ca2+ act as a 2' messenger?Δ in [Ca2+] in cytosol constitutes the signal
- 12^ [Ca2+]?Influx of EC Ca2+
Release from IC stores e.g. ER/SR/Mito - 13Decrease [Ca2+]?Ca2+ pumps sequester it back into organelle (remember the conc. in cytosol constitutes the signal).
- 14Structure and function of Calmodulin1. 4 Ca2+ binding sites
2. + When [Ca2+]>500nM
3. Ca-Calmodulin can bind + modify activity of target e.g. Calmodulin-dependent protein kinases (CAM Kinases) - 15Roles of CAM Kinases (4)Regulates fuel metabolism
Muscle contraction
Cell division
Inflammatory response - 16Kd[L] where 1/2 R is bound
Measure of binding affinity - 17Kd formula[R][L]/[RL]
- 18Deriving Kd from binding assayTotal Binding (A) - Nonspecific Binding (B)
=
Specific Binding (C)
1/2 [R] bound = Kd
Información
- Descripción
- El usuario ha eliminado su información de asignaturas Fichas sobre Cell Signalling II, creado por Usuario eliminado el 31/03/2018.
- Creado por
- Usuario eliminado · mar 2018
- Actualizado
- 31 mar 2018
- Visitas
- 14
- Historial
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- Creado por J yadonknow · 31 mar 2018
- Copiado por J yadonknow · 31 mar 2018
- Valoración