Mapa mental
Frozen section
Contenido del mapa
Frozen section
call attending + resident
- Add to LIS
- take section cut by resident
- Add freezing medium + freeze with OCT spray
- Cut 5 micro with cryostat
- put section on charged sticky slides
- immerse in absolute alcohol
- wash with tap water
- STAIN manually
- hematoxylin 1 MIN
- wash
- Acid alcohol 1 SEC
- wash
- Ammonia water 5 SEC
- wash
- Eosin 15 Sec
- DO NOT wash
- absolute alcohol 5 sec
- repeat 3 times
- Xylene x2
- Mount
- permount
- coverslips
- Label
- coverslips
- permount
- Mount
- Xylene x2
- repeat 3 times
- absolute alcohol 5 sec
- DO NOT wash
- Eosin 15 Sec
- wash
- Ammonia water 5 SEC
- wash
- Acid alcohol 1 SEC
- wash
- hematoxylin 1 MIN
- STAIN manually
- wash with tap water
- immerse in absolute alcohol
- put section on charged sticky slides
- Cut 5 micro with cryostat
- Add freezing medium + freeze with OCT spray
- take section cut by resident
- Add to LIS
Información
- Descripción
- Mapa Mental sobre Frozen section, creado por Ramez Kouzy el 15/07/2014.
- Creado por
- Ramez Kouzy
- Visitas
- 19
- Valoración