Test
Clone Identification and Expression
Preguntas del test (35)
1A radioactive probe can take what forms?
- AssDNA
- BRNA
- CDenatured dsDNA
- DOligonucleotides
2Probes can be
- ARadioactive
- BFluorescent
- CUse Ig's
3Name the two ways probes can be designed
- AGuesswork
- BCodon Bias
4What is codon bias?
- AWhere cells prefer one codon of an aa to another
- BWhere cells will reject certain codons due to what they contain
- CWhere some cells can only make tRNA's for certain codons
580% of Phe codons are TTC, 20% are TTT
- AVerdadero
- BFalso
6If the protein is an enzyme it can be found by adding the substrate
- AVerdadero
- BFalso
7State the possible forms of Ig's for protein separation?
- AImmobalised on a column
- BRemovable from solution
8Probes needn't be 100% accurate to work
- AVerdadero
- BFalso
9For a Western Blot, how is the DNA stuck to the membrane?
- ADenatured DNA sticks to membrane
- BBacteria are lysed to release DNA
- CDNA washes off
10Once the DNA has been bound, what happens next?
- AA probe is added
- BThe membrane is run through a gel
- CThe membrane is stained
11In an expression vector, where is the new gene added?
- AThe Multiple Cloning Site
- BThe Promotor Region
- CNext to the origin
12Name the parts on the vector
- AOrigin
- BAntibiotic Resistance
- CPromoter
- DTerminator
- EMultiple Cloning Site
13More compliated vectors have proteins tagged for purification
- AVerdadero
- BFalso
14What is involved in His Chain Purification?
- AThe addition of a His chain to the N terminus
- BThe removal of a His chain from the protein
- CThe use of a His chain to cut unwatned proteins
15His Purification uses Histadine's affinity for what metal?
- ANi2+
- BFe2+
- CNa+
16His-tagged proteins will bind to Nickel in a column. Others that don't bind will be washed off.
- AVerdadero
- BFalso
17The PET system involves using a promoter for the protein that you can control the expression of
- AVerdadero
- BFalso
18What promoter is used in the PET system to control protein expression?
- AT7
- BT8
- CT9
19In the PET system, what promoter is used to control the T_ expression?
- ALac promoter
- BSlo Promoter
- CPap promoter
20What is required for a good shuttle vector?
- AReplicable in both hosts
- BSelectable in both hosts
- CExpressable in both hosts
21Shuttle vectors are usually plasmids with additional sequences
- AVerdadero
- BFalso
22Shuttle vectors for yeast/fungi need
- ASpecial origin
- BAntibiotic/metabolic marker
23A baclovirus would be used as a vector into what?
- AInsects
- BPlants
- CMammals
24Secretion of certain proteins can be caused by what?
- ADisulphide bonds
- BAcetylation
- CMethylation
25Why are there two origins in the Yeast shuttle vector?
- AOne for the yeast to use
- BOne for the bacteria to use
26What is the method of selection in yeast?
- AURA3- allows growth in the absence of Uracil
- BTRA3- allows growth in the absence of thymidine
- CGRA3- allows growth in the absence of guanine
27Like bacteria, yeast cannot take up linear DNA
- AVerdadero
- BFalso
28What does a YAC contain that a plasmid does not?
- ANon-useful genes
- BYeast centromere
- CYeast telomeres
29Yeast can allow DNA to be directly inserted into its genome
- AVerdadero
- BFalso
30How can yeast accept DNA into its genome?
- AHomologous recombination
- BNon-homologous end joining
- CDirect injection
31In order to directly insert a gene, you must flank the gene with identical stretches of the part of the genome you want to insert it in
- AVerdadero
- BFalso
32Plants would use an aglobacterium T1 plasmid
- AVerdadero
- BFalso
33How do you replicate transformed plant cells?
- AGrown them as plantlets
- BClone them
- CAsexually reproduce them
34Mammal shuttle vectors are the same as yeast vectors, but the yeast origin is replaced with what?
- AAn SV40 promoter
- BA UG35 promoter
- CA FT12 promoter
35Retroviruses can also be used to insert transgenes into mammilian genomes by packing the transgene in a retroviral capsule
- AVerdadero
- BFalso
Información
- Descripción
- Year 2 Test sobre Clone Identification and Expression, creado por gina_evans0312 el 29/12/2013.
- Autor
- gina_evans0312
- Idioma
- English
- Visitas
- 514
- Etiquetas
- Valoración
- Aún sin valoraciones Valora este test