Test
Mutagenesis
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Preguntas del test (38)
1What is site directed mutation?
- AWhere mutagenesis is directed towards a specific site
- BWhere certain sites are more prone to mutagenesis than others
- CWhere certain sites cannot be mutated and must be worked around
2What form must the DNA be in for Site Directed Mutagenesis?
- AssRNA
- BssDNA
- CdsDNA
3The primer for site directed mutagenesis is designed to have one or more mismatches
- AVerdadero
- BFalso
4Once the mismatch has been achieved, how is it used?
- ADuring repair, the old base will be replaced with a new match
- BDuring replication, half the daughter cells will carry the mutation
5What is specific about the DNA used in the Kunkel Method?
- AIt's dsDNA
- BIt's ssDNA
- CFrom the Slo + form of E.Coli
- DFrom the dut ung. form of E.Coli
6What is Incorporated into the original DNA in order to make it susceptible to degrading
- AUracil
- BMethyl-cytosine
- CThymidine-3-phosphate
7Like with site directed, the primer annealed to the DNA is designed to create mismatches
- AVerdadero
- BFalso
8In both site directed and the Kunkel method, how is the primer extended?
- ADNA polymerase
- BTaq Polymerase
- CRNA polymerase
9Once the DNA has been inserted, what happens to the original DNA strand?
- AThe Uracil cause it to be partially degraded
- BThe Methyl-cytosine cause it to be partially degraded
- CThe Thymidine-3-Phosphate cause it to be partially degraded
- DBeing removed alone with the bp next to them
10The partially degraded strand is them re-synthesised by DNA repair proteins, finalising the mutation
- AVerdadero
- BFalso
11Which method has a way of determining which plasmid has the mutation?
- ASite- Directed
- BAltered Site
- CKunkel Method
12What is particular about the plasmid used in the Altered Sites method?
- AIt contains a non-functional Amp resistance gene
- BIt contains a gene for DNA repair proteins
- CIt's dsRNA
13What oligonucleotide primers are used during Altered Site mutagenisis?
- AMutant primer
- BAmpicillin 'Repair' primer
- CAmpicillin Non-Function primer
14What is the result, after DNA replication has occurred?
- AThe desired plasmid has the mutation, and tetracyline and ampicillin resistance
- BThe desired plasmid has the mutation and tet resistance but no ampicillin resistance
- CThe desired plasmid has the mutation and ampicillin resistance but no tetracyline resistance
15What causes the mutagenesis in the 'Quickchange' PCR method?
- AA two primers containing the mutation
- BRapid replication of the Site Directed mutant
- COne primer contains a mutation, the other is used to create the mutated plasmid
- DThe original DNA is then degraded, leaving only the mutated plasmid
16Why is Dpn used to degrade the original DNA?
- AIt degrades only methylated DNA
- BIt degrades only acetylated DNA
- CIt degrades only ibiquitinated DNA
17Site directed mutagenesis is unique in that no structural knowledge of the protein is required
- AVerdadero
- BFalso
18State the two methods by which non-directed mutation occurs in evolution
- AGradual accumulation of beneficiary mutations
- BGradual loss of unnecessary DNA
- CRecombination of genes to form hybrid protein
19These random mutations can be mimicked in a lab
- AVerdadero
- BFalso
20Random mutagenesis works best by introducing many mutations at the same time
- AVerdadero
- BFalso
21What will happen when this DNA replicates?
- AThe amino acid marked by the N's will be randomised
- BThe Leu will be deleted
- CThe N's are a stop codon, halting the gene
22Primers can be more specific and limit the choice of potential aa further
- AVerdadero
- BFalso
23Name an Error prone DNA polymerase
- ATaq
- BT7
- CPol
24Name the two conditions necessary for Error Prone mutagensis
- AAn error prone polymerase
- BConditions designed to increase error
- CNo DNA repair enzymes
- DRestriction enzymes
25DNA shuffling is used to create fusion proteins from two unrelated genes
- AVerdadero
- BFalso
26Why does gene shuffling work?
- ALigation repairs DNA errors
- BRelated genes have overlapping areas
- CRelated genes can ligate together with less error
27What protein is used to randomly cut up two related genes for DNA shuffling?
- ADNAase I
- BDNAase III
- CEcoRV
- DEcoRI
28The randomly cut up sequences are then ligated back together
- AVerdadero
- BFalso
29When creating hybrids, the traditional approach of screening colonies for hybrids is not efficient- so we use robots
- AVerdadero
- BFalso
30Name the two main methods of analysing mutants quickly
- ASurface display of proteins
- BElution
- CChemical compartmentalisation
31The advantage of Protein Surface Display in improved mutations is?
- AImproved mutations will bind more strongly to immobalised substrate
- BThey react more strongly to dyes
- CThey don't degrade as easily
32Once the variants are bound, they are washed so strongly only powerfully binding variants remain bound
- AVerdadero
- BFalso
33Once the most improved variation has been identified, what can happen to it?
- AIt's washed off and sequenced
- BIt's washed off and improved again
34Chemical Compartmentalisation uses an oil/acetyl emulsion
- AVerdadero
- BFalso
35Why are the droplets important?
- AThey make reading the DNA easier
- BEach droplet contains an individual mutation
- CEach droplet contains mutants that compliment each other
36The DNA cannot be transcribed or translated in the droplets, only sequenced
- AVerdadero
- BFalso
37Once the protein has been made, how are they assayed?
- ARobots put them on multi-well dishes for assay
- BFluorescent activated cell sorters are used
38How is fluorescence used for separation in Fluorescent Activated Cell Sorters?
- AThe more fluorescent product is produced, the better the enzyme
- BThe stronger the enzyme-substrate binding, the better the enzyme
- CThe more fluorescent substrate is used, the better the enzyme
Información
- Descripción
- Year 2 Test sobre Mutagenesis, creado por gina_evans0312 el 29/12/2013.
- Autor
- gina_evans0312
- Idioma
- English
- Visitas
- 1.014
- Historial
- Creado el 29 dic 2013 · actualizado el 16 nov 2016
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