476708
Description
Flashcards by sophietevans, updated more than 1 year ago
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Created by sophietevans
over 10 years ago
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| Question | Answer |
| What type of heavy chain exists in IgG? | Gamma. |
| Which types of light chain exist in IgG? | Kappa and lambda. |
| What is the purpose of the Ouchterlony technique? | Its purpose is to compare antigens (and their epitopes). |
| Which factors does the position of the precipitin line depend on? | Recognition by the antibody, the identity between adjacent antigens, and the concentration of the antigen. |
| Where is a precipitin line thickest? Why? | The precipitin line is thickest directly in between the antigen and complementary antibody because this is the closest point between them and so is the highest concentration of precipitin. The weaker concentrations are further out, so the precipitin line tapers off. |
| List some potential experimental errors that could result in unexpected patterns on the Ouchterlony slides. | The agar may not have been smooth, the pipetting technique may not have been correct which could mess up radial diffusion, and the wrong antigens could have been used. |
| What pattern is produced when there is identity (identical epitopes) between two antigens on an Ouchterlony slide? Why? | A continuous curve is produced between their radial diffusion overlaps, increasing the concentration that the antibody binds with, which moves the equivalence point further away from the wells, creating a curve. |
| What pattern is produced when there is no identity (both antigens are recognised by the antibody but they do not have identical epitopes) between two antigens? Why? | A cross is produced as, although there are two recognised antigens, they have two different epitopes and therefore the concentration is unaffected and the equivalence point unaltered. Two precipitin lines form and cross in accordance with their radial diffusion diameters. |
| What pattern is produced when there is partial identity (both have one epitope in common, but one has another epitope that the other doesn't have) between two antigens? Why? | A spur is produced. This is a result of the continuous curve formed by two antigens with identity increasing their concentrations and moving the equivalence point, and a straight precipitin line produced by the recognised epitope that only one of the antigens possesses. |
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