Lac Operon

involved in lactose breakdown in E.coli
1. if no lactose, a repressor is bound to operator, blocking transcription
  1. RNAP's binding to DNA is controlled
    1. binds at promoter
2. if lactose present, it binds to the receptor and causes a shape change such so it no longer binds to the operator and thus RNAP (Rna Polymerase) can transcribe the genes
E. coli
1. prefers glucose for energy
B-gal
1. used to cleave glycosidic bond in lactose
  1. when cleaved makes glucose and galactose
2. made by Lac z of the lac operon
Lac Y is gene for creating B-galactosidepermease
plac is sometimes called the promoter
Laci is the gene that encodes for the repressor protein
1. binds to the operator and represses function of this operon
2. Pi is promoter of Laci
  1. always made and binding to the operator
3. blocks RNA polymerase
4. Laci is bound to the DNA when lactose is not present
  1. when lactose is present it binds to the repressor which then falls off the repressor
Lac A is gene for transacetylase

Resource summary

	Created by izzy5605 over 10 years ago