Nutrients & O2, cell need to be in contact
with each other and this contact is signalling
chemically-so a mismatch/problem with
either signal or receptor will cause problems
humans loose between 50% & 75% of the cells
generated during cotrical development- through
programmed cell death=APOPTOSIS
Essential in normal development &
maintaining tissue homeostasis
Failure to regulate apoptosis= cancer, AIDS,
alzheimer's disease, parkinsons disease=
thought to be a result of deregulation of apoptosis
2 ways in which a cell can die: 1.
apoptosis 2. Killed by injurious agents
TNF- released from macrophages & initiate apoptosis.
Fas= TNF family= when binds to its receptor it activates apoptosis
SMACs released from mitochondria when an
increase in permeability- bind to apoptotic
inhibitor proteins (BCL2) in cytosol which usually
inhibit apoptosis therefore apoptosis can occur
Genetic considerations
It is possible to breed mice with inactivated CPP32 (apoptotic factor that promotes cell
death)- they have extra layers of cells in the retina & cortex which are usually removed by
apoptosis as it removes non essential cells- however the mice have inactivated CPP32
therefore this doesnt happen. The mice die because the gene is neurone specific
Death by injury
Cells that are damaged eg by mechanical
damage (e.g. radiotherapy can disrupt
nucli and promote p53=activator of
apoptosis) & exposure to toxic chemicals
Can be used to promote apoptosis when it is
failing to work- eg. chemotherapy-more than
one trigger/ multiple pathways for apoptosis
Caspase activation- inhibition of mRNA translation=
condensation of cell & organelles= chromatin
condensation, DNA fragmentation=loss of
membrane asymmetry= membrane remains
impermeable (form smaller packages= cell
blabbing)= cells fall apart into apoptotic bodies-
these are targetted for safe removal (by lysosomes)
Cytosol never exposed to extracellular fluid of healthy cells
Necrosis
Pro-inflammatory signalling &
cytokine production= swelling of
the cell and
organelles=chromatin
condensation= loss of mem
asymmetry= rapid loss of men
permeability= cell-membrane
explodes, remains stay
together= contents released=
inflammation of surrounding cells
What starts it?
1. Withdrawal of positive signals- growth factors for
neurons, interleukin 2 for mitosis 2. The receipt of
negative signals-oxidants, UV, X-rays, Chemotherapy.
Death activation factors (TNF, lymphotoxin & FasL)
Signals that arise within the cell
Internal signals
Healthy cells outer mem of mitoch express Bcl-2 on their
surface- Bcl-2 is bound to Apaf-1. Internal damage
releases Apaf-1=cytochrome c leaks out of mitoch. Apaf-1
+ cytochrome c bind to caspase9 forming the apoptosome=
then activates caspases 3, 6 & 7 (effector caspases)
p53 can also trigger activation of
caspases 8 & 10=causes
condensation of chromatin &
increases the permeability of
mitochondrial membrane causing
cytochrome C to leak out
CASPASES-family of proteases cleaving aspartic acid
residues. They cleave eachother so caspase 9 activates
other caspases=cascade of activation. Digestion of
structural proteins, DNA degradation & phagocytosis.
Once activated cant be deactivated- packaged as part of
blebbing process to protect other cells
p53 in cytosol can influence BCL2, Bax
interaction-BCL2 no longer inhibiting-Bax interferes
with mitochondrial mem & releases cytochrome c
External signals
Fas & TNF receptors are
integral mem proteins.
Binding of their respective
death activation factors
signals the activation of
caspase 8 and so on
FasL interaction with death domain on
internal face of membrane can lead to
destruction of mitoch/activation of
casphase 8- can then lead to DNA damage
& p53 release (further apoptotic signal)
Triggered by death activation factors
which bind to receptors at the cells surface
Death by suicide
cells shrink=mitchondria breaks down with the release of cytochrome
C= DNA degradation in their nuclei=break up into membrane wrapped
fragments= this exposes phospholipid phosphatidylserine
Phospholipid phosphatidylserine is normally hidden in the plasma men- when
exposed it is bound by receptors on phagocytic cells (macrophages)= they engulf
the cell fragments= phagocytic cells secrete cytokines these inhibit inflammation
Development
Resorption of a tadpole tail during metamorphosis, formation of fingers & toes
of the fetes requires removal of the tissue between them, sloughing off the inner
lining of the uterus during menstruation, proper synapse formation in the brain
Maintenance
Viral infection- cytotoxic T cells kill virus infected cells by induction of
apoptosis, defects in apoptotic machinery is associated with autoimmune
diseases, DNA damage leads to improper embryonic development & cancer
DNA damage=increased p53 produced (potent inducer of apoptosis)- mutated forms of the p53 genes are often
found in cancer cells-this permits the cell to live. Radiation & chemical used in cancer therapy induce apoptosis
Regulation of apoptosis could account for low neuronal
counts in patients with Down's syndrome because
BCL2 (cell survival protein) is lower in post-mortem
Down's patients, while Bax remains normal- Down's
patients gernerally develop Alzheimer's disease