What is the role of the Rb Susceptibility gene?
Nuclear transcription factor
What protein does Rb bind to?
What is the role of the protein bound to Rb?
What are the target genes of the Rb bound protein?
dNTP producing genes
Transcription factor producing genes
Growth factor producing genes
How is Rb phosphorylated and deactivated?
By a cyclin/CDK dimer
What protein phosphorylates CDK?
Which forms first during G1?
What causes the production of Cyclin/CDK E &D?
Which is required to activate E2F?
Name 3 CDK inhibitors
Which of the following binds to CDK, & twists its head to block the active site?
Which of the following bind to cdk with cyclin and physically block the active site?
How are the CDK inhibitors controlled?
Phosphorylation & degredation
Phosphorylation & activation
How long is the bp sequence p53 binds to?
Put the following p53 domains in order A- Signalling Domain B- Damaged DNA binding domain C-Oligomerization Domain D- Activation Domain E- DNA binding domain
How does p53 work in its active form?
As a monomer
As a dimer
As a tetramer
What does p53 bind to when active?
What can cause the activation of p53?
What does p53 do if activated in G1?
Reverts the cell back to G0
Forces cell through mitotic cycle
What happens if p53 is activated after G1?
Bax is transcribed- it initiates apoptosis
Bax is transcribed- It initiates S phase
Bax is transcribed- It inhibits M phase
Where do mutations causing non-function tend to occur in p53?
The DNA binding domain
The damaged DNA binding domain
The signalling domain
How is p53 removed from the cell?