Fluxograma
DISAGGREGATION OF PLANTS
Conteúdo do fluxograma
DISAGGREGATION OF PLANTS
PRIMARY AGGREGATION
MECHANICAL DISAGGREGATION
ENZYMATIC DISAGGREGATION
DISSECTION
FINE CHOPPING
WASH BY SETTLING
TRANSFER IN LAST WASH
REMOVE BSS
ADD THIN FILM OF MEDIUM
INCUBATE FOR 2-3 WEEKS
EXPLANT OUTGROWTHS
TISSUE SAMPLE
CELL TISSUE + DEBRIS
MORE CELLS + TISSUE DEBRIS
VIABLE CELLS + TISSUE DEBRIS
VIABLE CELLS + DAMAGED CELLS
PRIMARY ADHERENT CELL CULTURE
BIOPSY IN MEDIUM + ANTIBIOTIC
DISSECTION IN BSS + ANTIBIOTIC
CHOPPED TISSUE
COMPLETE MEDIUM + COLLAGENASE
DISAGGREGATED TISSUE + TISSUE RESIDUES
DISPERSED CELLS + RESIDUAL CULTURE OF EPITHELIAL ELLS
DISPERSED CELL SUSPENSION CONTAINS ENRICHED FIBROBLASTS
PRIMARY CELL CULTURE
ENRICHED EPITHELIAL FRACTION
PRIMARY CELL CULTURE
EDTA TREATMENT
COLLAGENSE
CENTRIFUGE
RESUSPEND
WASH
PRIMARY CELL CULTURE
Informações
- Descrição
- Fluxograma sobre DISAGGREGATION OF PLANTS, criado por Rashedha Camila em 27-12-2017.
- Criado por
- Rashedha Camila · dez 2017
- Atualizado
- 3 jan 2018
- Visualizações
- 31
- Avaliação