Genetic Engineering

Genetic Engineering

1. Procedure

   • RFLP
     • Applications
       • DNA fingerprinting
       • Disease Detection
       • Genomic Mapping
   • PCR
     • Step 2: Annealing of DNA Primers @ 55C
     • Step 1:Denaturation @ 95C
     • Step 3: Elongation of DNA @ 75C
   • Gel Electrophoresis
   • Nucleic Acid Hybridisation

2. Human Genome Project

   • Goals
     • Identify 25000 genes in human DNA
     • Store the information on DNA seq in publicly available domains
   • Benefits
     • Genetic Testing
     • Pharmacogenomics
   • Ethical Implications
     • Discrimination
     • COmercialisation and Patenting
     • Relaibility of Genetic Tests

3. Tools

   • DNA ligase
   • Restriction Enzymes
   • Vector
   • DNA Library
     • cDNA library
       • Features
       • Synthesis
     • Genome Library
       • Features
       • Synthesis

4. Protein Production

   • Process
     • Step 5: Extraction & Purification
     • Step 1: Isolation & Preparation of GOI
     • Step 2: Construction of recombinant DNA
     • Step 3: Transformation
     • Step 4: Selection and Identification
       • Blue/White colony screening
       • Replica plating
   • Human Growth Hormone
   • Insulin