Flashcards
Factors Affecting Enzyme Activity
Flashcards do conjunto (9)
- 1What factors affect enzyme activity?Temperature
pH
Enzyme concentration
Substrate concentration - 2How does temperature affect enzyme activity?More heat = more kinetic energy
Molecules move faster, increasing the frequency of successful collisions (substrates are more likely to collide with active sites)
Every enzyme has an optimum temperature - 3What happens if the temperature gets too high?The enzyme's bonds may break, changing the shape of the active site and the enzyme denatures
- 4How does pH affect enzyme activity?Above and below the optimum pH, the H⁺ and OH⁻ ions can break the ionic and hydrogen bonds that hold the enzyme's tertiary structure in place.
This makes the active site change shape and the enzyme denatures - 5How does enzyme concentration affect enzyme activity?The more enzyme molecules, the more likely a substrate is to collide, forming enzyme-substrate complexes
- 6What happens if the substrate concentration is a limiting factor?There comes a point when there are more than enough enzymes, so adding more enzymes has no further effect
- 7How does substrate concentration affect enzyme activity?The frequency of successful collisions increases, so more active sites are occupied and more enzyme-substrate complexes will form, until a saturation point
- 8What happens when enzyme concentration becomes a limiting factor?There are not enough active sites as they are all full
- 9What happens to substrate concentration over time and how does this affect enzyme activity?Substrate concentration decreases with time, and so the rate of reaction will decrease over time too.
Therefore, the initial rate of reaction is the highest rate of reaction
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- O usuário eliminou as informações de disciplina FlashCards sobre Factors Affecting Enzyme Activity, criado por Usuário eliminado em 05-03-2018.
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- Usuário eliminado · mar 2018
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- 5 mar 2018
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- Criado por Yinka F · 26 jan 2018
- Copiado por Yinka F · 5 mar 2018
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