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Papers covered; August 2011 May 2011

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Microorganisms MCQ's 2011

Questão 1 de 49

1

Modes of obtaining nutrition, used by at least some bacteria, include all of the following EXCEPT:

Selecione uma das seguintes:

  • Chemolithoautrophy

  • Photolithoautrophy

  • Chemoheteroautrophy

  • Chemoorganoheterotrophy

Explicação

Questão 2 de 49

1

Which of the following forms of oxygen are generally toxic to living organisms?

Selecione uma das seguintes:

  • Superoxide anion

  • Hydrogen peroxide

  • Hydroxyl radical

  • All of the above

Explicação

Questão 3 de 49

1

Carrier mediated transport is necessary when:

Selecione uma das seguintes:

  • Diffusion will not allow adequate amounts of a substance to enter the cell

  • Movement into the cell is against a concentration gradient

  • The level of nutrients in nature is very low

  • All of the above

Explicação

Questão 4 de 49

1

The size (as determined by the sedimentation factor) of the most useful RNA molecule for prokaryotic evolutionary studies is:

Selecione uma das seguintes:

  • 5S

  • 16S

  • 18S

  • 23S

Explicação

Questão 5 de 49

1

Which of these environmental factors influence microbial growth?

Selecione uma das seguintes:

  • pH

  • Water potential

  • Oxygen

  • All of the above

Explicação

Questão 6 de 49

1

The signature sequence is used extensively in the:

Selecione uma das seguintes:

  • Phylogenetic probe

  • Reclassification of misclassified organisms

  • Defining of a specific group within a domain

  • All of the above

Explicação

Questão 7 de 49

1

With regards to the bacterial genome which statement is the correct one?

Selecione uma das seguintes:

  • Bacterial genome is triploid

  • Bacterial genome is mostly haploid

  • Bacterial genome is mostly diploid

  • Bacterial genome is haploid

Explicação

Questão 8 de 49

1

With regards to bacterial plasmids which statement is the correct one?

Selecione uma das seguintes:

  • A plasmid is a regulatory gene

  • A plasmid is a short RNA molecule

  • A plasmid is an extra chromosomal DNA molecule

  • A plasmid is a non coding region found in some bacterial genomes

Explicação

Questão 9 de 49

1

All viruses:

Selecione uma das seguintes:

  • Have a genome that is a single molecule of nucleic acid

  • Are enveloped

  • Encode more proteins in their genomes than those contained in the mature virus particle

  • Have capsids that are icosahedral in shape

Explicação

Questão 10 de 49

1

Poliovirus:

Selecione uma das seguintes:

  • Has a double-stranded DNA genome

  • Has a capsid that is composed of 240 copies of a single protein

  • Replicates primarily in the upper respiratory tract

  • Belongs to the enterovirus genus of the picornavirus family

Explicação

Questão 11 de 49

1

Which of the following is NOT an alternative name for cutaneous fungal infections of humans:

Selecione uma das seguintes:

  • Superficial mycoses

  • Dermatophytes

  • Mycetoma

  • Ringworms

Explicação

Questão 12 de 49

1

Which of the following is NOT part of the plant disease triangle:

Selecione uma das seguintes:

  • Susceptible host

  • Climate change

  • Active pathogen

  • Suitable environment

Explicação

Questão 13 de 49

1

The Bacille-Calmette-Guérin (BCG) vaccine is:

Selecione uma das seguintes:

  • Heat-killed Mycobacterium tuberculosis

  • Attenuated live Mycobacterium tuberculosis

  • Mycobacterium bovis

  • Attenuated live Mycobacterium bovis

Explicação

Questão 14 de 49

1

Which of the following is a bacterial infection?

Selecione uma das seguintes:

  • Whooping cough

  • Polio

  • Acquired immunodeficiency syndrome

  • Malaria

Explicação

Questão 15 de 49

1

Pasteur is attributed with which one of the following discoveries?

Selecione uma das seguintes:

  • Was the first to see microorganisms under a microscope

  • First to discover and use agar

  • That there could be life without air

  • The first chemical antibiotics

Explicação

Questão 16 de 49

1

The length of a typical E. coli cell is approximately?

Selecione uma das seguintes:

  • 20 μm

  • 2 μm

  • 200 nm

  • 20 nm

Explicação

Questão 17 de 49

1

With respect to differences between members of the archaeal and bacterial domains/kingdoms, which is the correct statement?

Selecione uma das seguintes:

  • Both have peptidoglycan in their cell walls

  • Only some Archaea are capable of methanogenesis

  • Archaea are sensitive to β-lactam antibiotics

  • Bacteria have similar RNA polymerase to that of Archaea

Explicação

Questão 18 de 49

1

Which statement is correct with respect to the E.coli chromosome?

Selecione uma das seguintes:

  • It is negatively supercoiled

  • It is positively supercoiled

  • There are typically several origins of replication

  • It consists of over 50 million base pairs

Explicação

Questão 19 de 49

1

A culture of E. coli is diluted by a factor of 10-6 and, after plating out 100 μl on an agar plate, 93 colonies grew after incubation at 37oC overnight. The original culture therefore contained how many viable E. coli cells per ml?

Selecione uma das seguintes:

  • 9.3 x 10(7)

  • 9.3 x 10(8)

  • 9.3 x 10(6)

  • 9.3 x 10(5)

Explicação

Questão 20 de 49

1

Bacterial flagella bring about cell motion by which mechanism?

Selecione uma das seguintes:

  • Wave-like movement of the flagellum

  • Rotation of the cell

  • Rotation of the flagellum in one direction only

  • Rotation of the flagellum that is reversible

Explicação

Questão 21 de 49

1

In the Earth’s biosphere most fungal biomass and diversity is found:

Selecione uma das seguintes:

  • In the oceans

  • In soils

  • Under glaciers

  • In the atmosphere

Explicação

Questão 22 de 49

1

Fungal growth and cell division is NOT normally accompanied by:

Selecione uma das seguintes:

  • Biomass acquisition

  • Reduced vitality

  • Nucleic-acid replication

  • Enhanced ability to compete with other microbial species

Explicação

Questão 23 de 49

1

One factor that is NOT used to prevent or reduce fungal spoilage of processed foods is:

Selecione uma das seguintes:

  • Addition of benzene

  • Low water-activity

  • Refrigeration

  • Heat-sterilization

Explicação

Questão 24 de 49

1

Saprophytic activity is essential for the health of the biosphere because:

Selecione uma das seguintes:

  • It facilitates nutrient cycling and waste degradation

  • It kills host organisms and thereby averts overpopulation

  • It facilitates species evolution via endosymbiosis

  • It involves mineralization of inorganic rocks in the lithosphere

Explicação

Questão 25 de 49

1

The cardinal temperatures are the:

Selecione uma das seguintes:

  • Minimum temperatures

  • Optimum temperatures

  • Maximum temperatures

  • Minimum, optimum, and maximum temperatures taken together

Explicação

Questão 26 de 49

1

Carl Woese of the University of Illinois and his collaborators identified two major branches of prokaryotic evolution. What was the basis for dividing prokaryotes into two domains?

Selecione uma das seguintes:

  • Microscopic examination of the staining characteristics of the cell wall

  • Metabolic characteristics such as the production of methane gas

  • Ecological characteristics such as the ability to survive extreme temperatures

  • Molecular characteristics such as ribosomal RNA sequences

Explicação

Questão 27 de 49

1

Which prokaryotes should be expected to be most strongly resistant to plasmolysis in hypertonic environments?

Selecione uma das seguintes:

  • Extreme halophiles

  • Extreme thermophiles

  • Methanogens

  • Nitrogen-fixing bacteria that live in root nodules

Explicação

Questão 28 de 49

1

Photoautotophs use:

Selecione uma das seguintes:

  • Light as an energy source and CO2 as a carbon source

  • Light as an energy and methane as a carbon source

  • CO2 as both an energy source and a carbon source

  • All of the above

Explicação

Questão 29 de 49

1

Addition of blood to a culture medium only allows the haemolytic bacteria that grow on the plate to be picked out. This is an example of a:

Selecione uma das seguintes:

  • Selective medium

  • Differential medium

  • Complex medium

  • Synthetic medium

Explicação

Questão 30 de 49

1

A microbe growing in a functioning refrigerator is probably a:

Selecione uma das seguintes:

  • Psychrophile

  • Mesophile

  • Thermophile

  • Hyperthermophile

Explicação

Questão 31 de 49

1

Which one of the following sentences correctly describes the science of Metagenomics?

Selecione uma das seguintes:

  • Metagenomics is a genome- level study of the whole microbial community

  • Metagenomics is a genomics of industrially and medically important bacteria

  • Metagenomics is a study of genes’ expression

  • Metagenomics is a comparative analysis of the sequenced microbial genomes

Explicação

Questão 32 de 49

1

Genetic recombination is a:

Selecione uma das seguintes:

  • Change of DNA structure caused by radiation

  • Amplification of DNA molecules followed by cell division

  • Physical exchange between DNA molecules

  • Process resulting in protein synthesis

Explicação

Questão 33 de 49

1

All viruses:

Selecione uma das seguintes:

  • Enter cells by receptor-mediated endocytosis

  • Replicate their genomes in the nucleus of the cell

  • Replicate their genomes in the cytoplasm of the cell

  • Surround their progeny genomes with capsid proteins inside infected cells

Explicação

Questão 34 de 49

1

Tobacco mosaic virus has:

Selecione uma das seguintes:

  • An icosahedral capsid

  • A helical capsid

  • Multiple copies of each of four proteins making up the capsid

  • Multiple copies of two proteins making up the capsid

Explicação

Questão 35 de 49

1

Anthrophilic dermatophytes (fungi causing cutaneous infections of humans) are primarily associated with:

Selecione uma das seguintes:

  • Soil

  • Animals

  • Plants

  • Humans

Explicação

Questão 36 de 49

1

Secondary symptoms of infections of plants by pathogens:

Selecione uma das seguintes:

  • Occur at some distance from the point of infection

  • Normally occur close to the site of active infection

  • Are an ideal place to isolate the causal organism

  • Never occur at the same time as primary symptoms

Explicação

Questão 37 de 49

1

Which one of the following infectious diseases has been eradicated from the human population?

Selecione uma das seguintes:

  • Plague

  • Leprosy

  • Small pox

  • Polio

Explicação

Questão 38 de 49

1

Which of the following is a host component important in specific immune recognition of extracellular microbes?

Selecione uma das seguintes:

  • Antigen

  • Antibody

  • Phagocyte

  • Lipopolysaccharide

Explicação

Questão 39 de 49

1

With regard to the differences between bacterial and eukaryotic cells which statement is the CORRECT one?

Selecione uma das seguintes:

  • Bacterial cells do not contain mitochondria

  • Bacterial cells are always larger than eukaryote cells

  • Bacteria do not contain a chromosome

  • Eukaryotic cells are sensitive to β-lactam antibiotics

Explicação

Questão 40 de 49

1

Koch’s postulates most CORRECTLY relate to which one of the following statements?

Selecione uma das seguintes:

  • The isolation of a bacterium in culture from an infection alone indicates that it caused the disease

  • Isolation and cultivation of a microorganism from a diseased animal, then inoculation into another healthy animal to cause the same disease, followed by re-isolation of the same microorganism, indicates that it is the cause of the disease

  • The observation of virus particles in tissue from a human with disease indicates that it has caused the disease

  • Inoculation of a bacterium into an animal and observation of disease alone indicates that it is the cause of the disease observed

Explicação

Questão 41 de 49

1

Which statement is CORRECT with respect to Lipopolysaccharide (LPS)?

Selecione uma das seguintes:

  • LPS is found in Gram positive cells

  • LPS is found in Gram negative cells in the cytoplasmic membrane

  • LPS is found in the outer-membrane of Gram negative cells.

  • LPS is found inside all bacterial cells

Explicação

Questão 42 de 49

1

A culture of E. coli is diluted by a factor of 10-6 and, after plating out 100 μl on an agar plate, 57 colonies grew after incubation at 37oC overnight. The original culture therefore contained how many viable E. coli cells per ml?

Selecione uma das seguintes:

  • 5.7 x 10(7)

  • 5.7 x 10(8)

  • 5.7 x 10(6)

  • 5.7 x 10(5)

Explicação

Questão 43 de 49

1

With respect to differences between members of the archaeal and bacterial domains/kingdoms, which is the CORRECT statement?

Selecione uma das seguintes:

  • With respect to differences between members of the archaeal and bacterial domains/kingdoms, which is the CORRECT statement?

  • Only some Archaea are capable of methanogenesis

  • Archaea are sensitive to β-lactam antibiotics

  • Bacteria have similar RNA polymerase to that of Archaea

Explicação

Questão 44 de 49

1

Which statement is CORRECT with respect to peptidoglycan?

Selecione uma das seguintes:

  • There are multiple molecules of peptidoglycan in bacterial cell walls

  • Only Gram positive cells possess peptidoglycan

  • There is only a single molecule of peptidoglycan in a bacterial cell wall

  • Peptidoglycan is only found in Gram negative cell walls

Explicação

Questão 45 de 49

1

The primary reason that health professionals recommend that new-born babies are not washed is:

Selecione uma das seguintes:

  • So that they can be inoculated with symbiotic yeast species

  • To avoid imbalances in the microbial ecology of indigenous flora

  • To avoid accidental contamination with fungal spores

  • So that clinical microbiologists can study the impact on the current generation

Explicação

Questão 46 de 49

1

Post-genomic techniques include:

Selecione uma das seguintes:

  • Proteomics

  • Use of restriction enzymes

  • Gene sequencing

  • Studies of signal-transduction pathways

Explicação

Questão 47 de 49

1

Systems biology approaches to the study of fungal cells do NOT necessarily involve:

Selecione uma das seguintes:

  • Generation of data from biological experiments

  • Mathematical quantification of changes in cell components

  • Creation of a model of the cellular system in silico

  • Creation of a conceptual model based on philosophical techniques

Explicação

Questão 48 de 49

1

Which of these statements is untrue?

Selecione uma das seguintes:

  • Some species of yeasts are highly tolerant to ethanol stress

  • Some species of fungi are extremely xerophilic

  • Many fungi are more tolerant to water stress than any bacterial species

  • Fungi can grow over the entire pH range from 0 to 14

Explicação

Questão 49 de 49

1

An extremophilic microbe is one which:

Selecione uma das seguintes:

  • Can survive dry conditions

  • Can grow only when there is a mixture of stress parameters (e.g. high temperatures and low pH)

  • Can grow optimally under extreme conditions

  • Becomes dormant under extreme conditions

Explicação