Quiz
MCB Midterm Summary
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Perguntas do quiz (28)
1A/an _____________ vector quantifies the expression of a gene to measure the activity of regulatory elements.
- AReporter
- BExpression
- CCloning
2An expression vector is used to _________.
- AStudy the effect of overexpression of a gene on cell functions
- BMeasure activities of regulatory sequences
- CQuantify the expression of a gene downstream of the regulatory sequence of interest
- DAmplify and isolate the product of a gene
3Which of the following is NOT an essential component of a plasmid?
- AOri
- BAntibiotic resistance gene
- CMultiple cloning site
- Dlac operon
4What would not be found in a eukaryotic expression vector?
- ABacterial Ori
- BAmpicillin selectable marker
- CEukaryotic promoter
- DMCS
- EPolyA signal
- FNeomycin - eukaryotic selectable marker
- GTransfection factor
- Hluciferase gene
5The expression of the _______ gene is quantified to study the activity of a regulatory sequence. In which type of vector would you find this gene?
- Aluciferase - expression vector
- Bluciferase - reporter vector
- ClacZ - expression vector
- DlacZ - reporter vector
6Which is NOT involved in the construction of cDNA libraries?
- Areverse transcriptase
- Boligo dT primer
- Cribonuclease H
- DDNA polymerase
- EDNA ligase
7What is the role of ribonuclease H in the synthesis of cDNA?
- ADegrade the mRNA template
- BSynthesize the mRNA template
- CLIgate the 2 strands of newly-synthesized DNA
- DSynthesize the first strand of cDNA from mRNA template
8Which type of vector is pBluescript?
- AExpression because it tests for the expression of lacZ gene
- BCloning because it is used to isolate DNA sequences with a built-in mechanism to select for recombinant plasmids
- CReporter because it quantifies lacZ expression
9Which outcome indicates that a colony on the pBluescript agar contains the recombinant plasmid?
- AColony is blue
- BColony is white
- CThere is a clearing zone around the colony
10Which is NOT true about enhancers sequences?
- AAlways located upstream of a gene
- BFunction in both 5'-3' and 3'-5' directions
- CInfluence expression of genes that are physically distant from the enhancer
- DFunction is not affected if location is moved
11Which of the following are DNA binding motifs?
- AHelix-turn-helix
- BZinc finger
- CLeucine zipper
- DHelix-loop-helix
12_______ hormones bind hormone response elements (HRE) in the nucleus to upregulate target genes.
- ASteroid
- BPeptide
13Epigenetics (changes in chromatin structure) is heritable and irreversible.
- AVerdadeiro
- BFalso
14Position-effect variegation is the change in gene expression caused by the translocation of genes between heterochromatin and euchromatin regions.
- AVerdadeiro
- BFalso
15Which does NOT result from histone acetyltransferases (HATs) activity?
- ALoosening of DNA packaging
- BDecreased gene expression
- CEasier transcriptional factor binding
- DMore open chromatin
16Which would lead to a decrease in gene expression?
- AHAT
- BHDAC
- CDNA methyltransferases
- DSWI/SNF
17What is the difference in dosage compensation of Drosophila and C. elegans?
- ADrosophila: hyperactivation of male X; C. elegans: hypoactivations of both female X'es
- BDrosophila: hypoactivations of both female X'es; C. elegans: hyperactivation of male X
- CDrosophila: inactivation of a female X; C. elegans: hyperactivation of male X
- DDrosophila: hyperactivation of male X; C. elegans: inactivation of a female X
18Imprinted gene is the inheritance of parental acetylation pattern.
- AVerdadeiro
- BFalso
19Which does NOT result in increased mRNA stability?
- AelF4E
- BPABPI
- CDeadenylase
- DLong poly-A tail
20______ removes the hairpin loop of the Pre-miRNA to form ds siRNA.
- ADrosha
- BDicer
- CExp5
- DAgo2
21G1/S checkpoint is controlled by [blank_start]Rb[blank_end].
DNA damage checkpoint is controlled by [blank_start]p53[blank_end].- ARb
- Bp53
- Cp21
- DRas
22Which is NOT correct regarding cell cycle control?
- APhosphorylation of Rb leads to growth factor independent G1/S progression.
- BA loss-of-function mutation in the p53 gene leads to a rapid accumulation of pro-cancer mutations.
- CCyclin D is an inhibitor of Rb, which in turns is an inhibitor of E2F.
- Dp53 upregulates BAX which activates the extrinsic apoptotic pathway.
23The p53 gene is mutated in >50% cancers.
- AVerdadeiro
- BFalso
24Which gene is the most closely-associated with chronic myelogenous leukemia?
- Abcr-abl
- Bc-myc
- CRas
- DHER2
25In Burkitt's lymphoma, ______ gene is translocated to a region controlled by a strong enhancer.
- Ac-myc
- Bbcr-abl
- CHER2
- DRas
26Which does NOT result in growth factor independent G1/S transition?
- AHyperphosphorylated Rb
- BLoss-of-function mutation of Rb
- CMutation in E2F which prevents Rb binding
- DLow levels of cyclin D leading up to DNA duplication
27Which of the following cellular properties is the LEAST likely to cause cancer?
- AMutations in signalling proteins and receptors
- BInsensitive to anti-growth signals
- COverexpression of pro-apoptotic proteins
- DOverexpression of telomerase
- EUpregulation of VEGF
- FAbility to metastasize and invade other tissues
28[blank_start]Ras[blank_end] hyperactivation results from a point mutation to Valine and GTP-independent activity.
[blank_start]bcr-abl[blank_end] fusion protein is closely associated with chronic myelogenous leukaemia.
[blank_start]HER2[blank_end] is an epidermal growth factor receptor, whose oncogenic properties arises from gene amplification.
In Burkitt's lymphoma, [blank_start]c-myc[blank_end] gene is translocated to a region controlled by a strong enhancer. It is also amplified in childhood neuroblastomas.- ARas
- Bbcr-abl
- CHER2
- Dc-myc
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- O usuário eliminou as informações de disciplina Quiz sobre MCB Midterm Summary, criado por Usuário eliminado em 04-12-2019.
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- Criado em 4 dez 2019 · atualizado em 4 dez 2019
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