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Microbio 3

brittbartak Englisch 61 Karten

Microbio 3

Karten in diesem Stapel (61)

  1. 1CFU is a unit that describesBacterial living cell density
  2. 2A plate with ___ colonies can be recorded as TFTC,Fewer than 30
  3. 3Plate with ___ can be counted as TNTCMore than 300
  4. 4What unit is used for density of viral growth?PFU
  5. 5You dilute a bacteria culture by a factor of 10^4 and spread 200ul on a plate. The next day you count 150 colonies on the plate. What was the original cell density?7.5 x 10^6 CFU/ml
  6. 6How can a 1000-fold dilution be written?1/1000, 1X10^-3, 10 X 10 ^ -4
  7. 7Why is serial dilution performed?To attain a countable number of colonies
  8. 8What did we use to sterilize the hockey stick spreaders in this lab?Ethenol and bunsen burner
  9. 9When should a student remove glowvestouching door handle to the room
  10. 10T/F we did a bacteria spread on 2 plates for each dilution to obtain reliable numbers?T
  11. 11T/F on tuesday, we serialy diulted our bacteria culture 10-fold five times?False
  12. 12What is bacteria transformation?Bacteria gets genetic information from enviornement
  13. 13Plasmids are sometimes beneficial to bacteria becauseplasmids may contain antibiotic resistant genes
  14. 14What is an operon?Structural and functional genetic units of prokaryotes
  15. 15Green flourescent protei nused as indicator for gene transcription
  16. 16PlasmidUsed as vector for GFP gene to E.coli
  17. 17Regulatory molecule for arabinose promotor. Allows RNA to bindAntibiotic resistance gene
  18. 18Enzyme that hydrolyzes certain antibiotics. differentiates cells.Antibiotic resistant gene
  19. 19T/F following transformation, the plasmid becomes intergrated into bacteria chromosomes?False
  20. 20T/F we used plasmid pGLO as a vector to carry GFP protein into bacteriaFalse
  21. 21A complete arabinose operon consists ofpromotor and 3 structural genes
  22. 22In an emergency room at the lawrence hospital, which of the following would likely be the biggest drawback to identifying bacteria species using API-20E prodecure we used in class?Long test incubation tome/wait for results because it takes 24 hours
  23. 23After 24 hours of the API20 test there is no growth. What can you assume about fermentation of nitrate reduction of the test? Is it a member of enterovacteriaceae?Negative for nitrate fermentation and not enterobactericae
  24. 24Why is agar used?Solidifying agent
  25. 25Which of the following contained or "displayed" epitopes that recongized in agglutination reaction?The antigen
  26. 26What are antibodies primarily composed of?Protein
  27. 27Which of the following are composed of double stranded DNA?pGLO plasmids
  28. 28What was the green flurocent protein (GFP) composed of?Amino acids
  29. 29What is the pGLO plasmid composed of?Nucleotides
  30. 30After E.Coli sucessfully transformed using the pGlo plasmid, where is the gene for GFPInside E.coli but not in bacterial genome
  31. 31In a typical pGlo transformation procedure, appoximatley what percent of bacteria undergo calcium and heat shock actually end up transformed?<1%
  32. 32Calcium and heat shock treatment caused ___ in our transformation expirimentpores to form, allowing uptake of DNA
  33. 33Which type of colonies would most likely to produce GFFP?pGlo-transformed E.coli cultured in presence of amipicillin + Arbinose
  34. 34Why were the vials ( or transoformed cells) incubated for 10 min in LB nrothe rather than transferring their contentes directly to plates?Allow ampicillin-resistence protein to be produced
  35. 35What can be fermented?polysacchardies
  36. 36Which is true about selective media?-aids in isolation
    -produced by adding bacteriostatic agent
    -produced exculding number of nuitrent sorces from media
    -colonies appear isolated could have unseen contaminants
  37. 37Suppose a student testing an unknown culture performs the DNAse test according to the protocol; after 48 hours, the DNAse media has not clarifie d. Which of the following conclusions would be most proper / justified?No DNAse activity observed
  38. 38The locations where chemically induced DNA replications error occur are?Randomly distributed
  39. 39How would you expect chemical TOXICITY to affect growth of Ames - strain Salmonella typhimurium ?Prevent growth
  40. 40How would you expect chemical MUTAGENICITY to affect growth of Ames - strain Salmonella typhimurium ?Induce mutations, reduce auxotrophs
  41. 41. Which list is ordered from smallest to largest (in ter m s of physical size)?gene, operon, plasmid, porkaryotic, chromosome, eukaryotic chromosome
  42. 42What is the fundamental, underlying reason why species of bacteria vary with respect to their abilities to utilize different types of carbohydrates?DNA- Fermentation processes are catalyzed by genetically-encoded enzymes
  43. 43Which of the following factors could be rate - limiting with respect to the rate of bacterial population growth?spatial constrants/compeition, depletion of substrates, starting materials, accumilation of bacteria metabolities, and length
  44. 44At temperatures ABOVE the cardinal temperature of a bacteria l species , the rate of cell division _____________; at temperatures BELOW that cardinal temperature, the rate of cell division ____________Decresase and decrease
  45. 45Concentration (cell density) of bacterial in suspension may be estimated using a spectrophotometer. Increasing turbidity is the same as ____________optical density; both indicate _______________ cell densityincreasing, and decreasing
  46. 46—It is mutagenicIt is mutagenic
  47. 47—Has most concentration of chemical testingHas most concentration of chemical testing
  48. 48In a typical Ames test procedure , approximately what percent of mutations result in rescue of auxotrophic mutants?Very few - less than 1%
  49. 49"Which plate(s) do you need to examine to determine if your test substance is mutagenic?"Minimal media plate
  50. 50"Why can't your test substance [the potential mutagen] contain protein?Bacteria could obtain histidine from test substance
  51. 51The tests we use in this class for differentiation of species during the mixed double unknown project wereQualitative
  52. 52Were the blue nitrile gloves you used in lab sterile ?No
  53. 53Aggluination is an interaction between?Antigen and antibody
  54. 54What is contained in the various regents added to strep sapp during aggulationantibody
  55. 55Which of the following is a good negative control for grape juice fermentation?Grape juice
  56. 56What is a possible byproduct of fermentation?Lactic acid and co2
  57. 57what is the range of acceptable colonies for a standard plate count?30-300
  58. 58What is the end zone of inhibition called?minimum inhibitory concentration
  59. 59Which plate would you expect to see GFP (glowing) colonies?LB + ampicillin + Arbinose
  60. 60Which antibiotic is most effective against antimicrobial agent?10 micrograms of antibiotic and 20 mm zone of inhibition
  61. 61t/f bacteria resistance to specific antibiotics change over time?True

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Karteikarten am Microbio 3, erstellt von brittbartak am 05/05/2014.
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