Karteikarten
Replication of DNA
Karten in diesem Stapel (11)
- 1What are the two main stages of cell division?nuclear division and cell division
- 2What must occur before a cell divides?It's DNA must be replicated in order for the daughter cells to contain enough genetic information to produce enzymes and other substances they may need.
- 3Why is DNA semi-conservative?50% is from the original DNA strand and 50% is from a new DNA strand
- 4What type of bonds are between the four organic baseshydrogen bonds between A and T as well as between G and C
- 5What enzyme is used to break down the hydrogen bonds and which enzyme is used to put them base pairs together again?DNA helicase- unzips the genes
DNA polymerase- puts the genes back together - 6Explain the steps to DNA replication.helicase breaks down hydrogen bonds, double helix separates, polynucleotide strand is then joined with other nucleotides via DNA polymerase to form 50% new and 50% original strand.
- 7If an inhibitor of DNA polymerase were introduced into a cell, explain what the effect would be on DNA replication.The linking together of new nucleotides could not take place. While the nucleotides would match up to be complementary, they would not join together to form new strands.
- 8What's the evidence for semi-conservative replicationWatson and Crick used DNA that contained Nitrogen- some 14N and some 15N
- 9Why was bacteria chosen to be used for this experiment?It divides rapidly and you can extract their DNA easily (via centrifugation)
- 10What happens with the medium containing 14N and 15N bacteria14N is light so will float
15N is heavier so will be closer to the bottom - 11What happens when 14N and 15N grow and allow to replicate once?They are bound together and form in between where the 15N and the 14N formed.
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- AS Biology2 (The Cell Cycle) Karteikarten am Replication of DNA, erstellt von sammi_taylor01 am 29/05/2013.
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- sammi_taylor01 · Mai 2013
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- 19 Feb 2016
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