Karteikarten
Micro Lab Quiz 8
Karten in diesem Stapel (18)
- 11. Blood hemolysisa. Differential test with basis of differentiation being the ability to lyse RBC’s (red blood cells)
- 2Blood Hemolysis Indicatorpart of the medium- the RBC themselves
- 3Blood Hemolysis Resultsi. Alpha – partial hemolysis (green discoloration around growth)
ii. Beta – full hemolysis (yellow halo)
iii. Gamma – no hemolysis (red touching growth) - 42. Phenylalanine deaminasea. Phenylalanine is an amino acid that becomes phenylpyruvic acid and ammonia
- 52. Phenylalanine deaminase indicatorFerric Chloride (FeCl3)
i. Reacts with phenylpyruvic acid to produce a green color
ii. Ferric chloride is not a pH indicator
1. Grabs product, forms complex - 62. Phenylalanine deaminase Resultsi. Green is positive
ii. No color change is negative - 7Decarboxylationa. Differential test based on presence of decarboxylating enzymes
i. Lysine decarboxylase
ii. Arginine decarboxylase
iii. Ornithine decarboxylase - 8Decarboxylation how it worksb. AA --> CO2 is released
c. Decarboxylation increases the pH of the environment
d. These enzymes are inducible enzymes - 9Decarb enzyme inducing requirements (1)1. Acidic environment
a. Created via fermentation of glucose
i. Organisms that are prompted to ferment because of the anaerobic environment in which they’re placed by the addition of mineral oil - 10Decarb enzyme inducing requirements (2)2. The AA that the enzyme works on
- 11Decarb indicatorBromcresol purple
- 12Decarb resultsi. Positive result: purple (basic)
ii. Negative result: yellow (acidic)
1. Fermentation is taking place, but there’s no decarboxylating enzymes made by the bacteria
iii. Brown is the color when there’s no color change (neutral) - 13PYRa. Differential test for ability to hydrolyze L-pyrrolidonyl-Beta-naphthylamide (PYR)
i. This is performed by the enzyme pyrrolidonyl peptidase
b. PYR can be hydrolyzed into L-pyrrolidone carboxylic acid and Beta-naphthylamine
i. Beta-naphthylamine reacts with the p-dimethyl-aminocinnamaldehyde on the slide to form the pink color - 14PYR indicatorp-dimethyl-aminocinnamaldehyde
- 15PYR resultsi. Formation of a pink color within 1 minute is a positive result
ii. Absence of a pink color after 1 minute is a negative result - 16Bile esculina. Differential test based on the presence or absence of the ability to hydrolyze esculin in the presence of bile salts
i. Esculin is hydrolyzed into esculetin and glucose - 17Bile esculin indicatorb. Indicator is Fe3+, formed from the ferric citrate in the agar
- 18Bile esculin resultsi. Formation of a dark brown or black color is positive
ii. No color change is negative
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- Karteikarten am Micro Lab Quiz 8, erstellt von tawnee.keel am 09/11/2015.
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