Flussdiagramm
Ohne Titel
Inhalt des Flussdiagramms
STEPS INVOLVED IN CELL CULTURE
Disaggregation of Explant
PRIMARY EXPLANT TECHNIQUE
MECHANICAL DISAGGREGATION
ENZYMATIC DISAGGREGATION
EDTA TREATMENT
Dissection
Fine chopping
Wash by settling
Tissue sample
Cell + debris
More cell +debris
Viable cell+debris
Chopped
Washed
Cold trypsinization
Tissues in vials
Collagenase
Centrifuge
Resuspend
Wash primary cell culture
Warm trypsinization
Transfer to BSS
Remove to BSS
Dilute the cells
Add thin film of medium
Incubate for 2-3 weeks
Incubate
Primary adherent cell culture
Explant out growth
Place on ice for 1.6 hrs
Disaggregate cells+ tissue residues
Incubate 335.6 degree 20-30 min
Tissue pieces+ 100ml trypsin
Pieces +trypsin
Dispersed cells in medium
Incubate 35.6 degree for 48-72 hrs
Dispersed cells
Primary cell
Subculture
Cell line
Informationen
- Beschreibung
- Flussdiagramm am Ohne Titel, erstellt von Salma Jaffar am 08/01/2018.
- Erstellt von
- Salma Jaffar
- Aufrufe
- 9
- Bewertung