Protein Assembly, Dynamics & Function

Protein Assembly, Dynamics & Function

1. Protein Folding

   • Unfolded State
   • Molten Globules
   • Levinthal Paradox
   • Folding Funnels & Models
     • Hydrophobic Collapse
     • Framework
     • Nucleation-Diffusion
   • Protein Folding Experiments
     • Anfinsen's Experiment
     • Detection of Folding Intermediates
     • Computational

2. Protein Dynamics

   • Types of Motions
   • Electron Paramagnetic Resonance
   • Pulsed EPR
   • Pulsed Electron Electron Double Resonance

3. NMR Spectroscopy

   • Coupling
     • J-coupling
     • Dipolar coupling
   • Use in Structural Biology
   • Case Studies
     • Calmodulin
     • N-PGK
     • BPGM

4. Membrane Proteins

   • Integral Membrane Proteins
   • Ion Channels
   • ABC Transporters

5. Molecular Machines

   • Brownian Ratchet?
   • SecYEG Translocation Channel
   • Bacteriophage DNA Packaging Motors
   • Mitochondrial ATP Synthase
   • Bacterial Flagella

6. Protein Assembly

   • Classes of Chaperones
     • Chaperones
     • Polo Mint
     • Scissors
     • Paper
     • Stone
   • Assembly of Multisubunit Complexes
     • Bacterial Ribosome
     • Motifs
     • ABC Transporter
     • Art MP & Art J
     • MalFGK