Chromatography

Chromatography

1. Technique 4 separation/identification of molecules based on differential affinity

   • Can be used for qualitative testing
     • Qualitative- how much substance you have
       • Differential Affinity - to attach to something (weak Bond)
         • Strong Bond - are Stable
           • Weak Bond- Hydrogen- Hydrophobic = Flexibility
             • DNA-2 Strands= Flexible
       • 2 Phases of Chromatography
         • 1st- Stationary (Cellulose paper) can be known/unknown
           • 2nd- Mobile ( Liquid) Solution will pass over stationary
             • Separation = Decanting
               • Each samples has their own differential affinity
                 • Affinity can identify chemical and allows you to identify them as well
                   • Capillary Action- movement of fluid, up thin glass
                   • Adhesive- unlike molecules
                     • Cohesive- same molecules
                       • Water has both Cohesive/Adhesive properties
                 • Cohesive Color moves up together
                   • Colors adhere to paper