Generation of Ureteral Mucosa By Tissue Engineering

Generation of Ureteral Mucosa By Tissue Engineering

1. Scaffold

   • Cell Cultures: Coculture
     • hADSC
     • Urothelial cells
   • Mesh
     • PLLA
     • Collagen endolumial stents

2. In Vitro Assessment

   • MTT: 1,3,5,7 d
   • Optical microscopy and SEM
   • Immunohistochemical staining antycitokeratin 19 antibodies
   • Expresssion of CK-18 and UP2

3. In vivo Experiments: Grafts in athymical mice

   • 14 days and (2 month, if possible) after implantation
     • Immunofluorescence: NuMA presence
     • H&E stainig: cell infiltration
     • Optical microscopy and SEM
     • Expression of CK-18 and UP2

4. Tatiana Fokina, 2A

5. Bibliografia

   • Ross Histologia texto y Atlas. Correlacion con biologia molecular y celular. Wojciech Pawlina. ISBN 978-84-16004-96-6
   • Recent Advances in Ureteral Tissue Engineering. Paul K. J. D. de Jonge,corresponding author Vasileios Simaioforidis, Paul J. Geutjes, Egbert Oosterwijk, and Wout F. J. Feitz
   • New ureteral scaffold constructed with composite poly(L-lactic acid)–collagen and urothelial cells by new centrifugal seeding system† Authors Wei-Jun Fu, Yong-De Xu, Zhong-Xin Wang, Gang Li, Jian-Guo Shi, Fu-Zhai Cui, Yuanyuan Zhang, Xu Zhang
   • Tissue engineering of ureteral grafts by seeding urothelial differentiated hADSCs onto biodegradable ureteral scaffolds† Jian-Guo Shi, Wei-Jun Fu, Xiao-Xiong Wang, Yong-De Xu, Gang Li, Bao-Fa Hong, Yan Wang, Zhi-Yan Du, Xu Zhang
   • Biomaterials and Tissue Engineering in Urology. J Denstedt, A Atala. ISBN 1845696379, 9781845696375