CALU-3

CALU-3

1. HISTORY (details of donor)

   • Name of depositor: J Fogh Deposited as: Homo sapiens Age: 25 years Gender: male Ethnicity: Caucasian Disease: adenocarcinoma The patient had received prior therapy with cytoxan, bleomycin and adriamycin. Blood Type A; Rh+

2. CULTURE CONDITIONS

   • subculture recommendations
     • Maintained in a 1:1 mixture of Ham's F12, DMEM, 10% fetal bovine serum supplemented with 100 μg/ml penicillin G (Sigma), 100 μg/ml streptomycin sulfate (Sigma), cells were plated in 150 cm2cell culture flasks and subcultured before reaching confluency using a 0.25% trypsin solution in EDTA, culture medium was changed every 2 days, cells were split 1:2 during each passage.
   • method of dissociation
     • Well-differentiated human bronchial cell line with the characteristics of the serous cells of airway submucosal glands. The submucosal glands play a major role in mucociliary clearance because they secrete electrolytes that facilitate airway hydration.
   • supplements
     • Calu-3 cells were grown using air-interfaced condition (AIC) on polyester cell culture supports. Monolayers were evaluated for transepithelial electrical resistance (TEER), permeability to the paracellular marker fluorescein sodium (flu-Na), surface P-gp expression, and functionality. Mucous secretion was also identified by alcian blue staining
   • culture medium
     • Put them in a medium with higher serum concentration
   • mode of growth
     • Have characteristics of both serous and mucus cells, can be cultured as a flat sheet, and respond to secretagogues that regulate the glands in vivo. They form polarized monolayers with tight junctions and express several acinar cell markers.

3. TYPE OF CELL LINE

   • continuous
   • transformed

4. GROWTH CHARACTERISTICS

   • growth rate
     • Continuously propagated, grew rapidly and consistently, and were passaged approximately once a week
   • morphology
     • Monolayers, cuboidal and polygonal cells, microvilli on the apical surface
   • plating efficiency
     • Let the cells grow on the top of feeder cells. Plate irradiated 3T3 cells the day before plating Calu3 cells. They grow much better .
   • cell viability
     • It is based on cellular mitochondrial dehydrogenase (MDH) activity, which measured by MTT reduction and conversion to blue formazan salt that was quantified after extraction from cells.

5. SPECIES

   • Homo sapiens

6. DEFINITION

   • A human lung cancer epithelial cell line commonly used in cancer research and drug development
   • references: https://www.atcc.org/products/all/HTB-55.aspx#characteristics

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