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1. Anatomy of the female reproductive system

   • Physiology and hormonal changes during menstrual cycle
   • Normal Microbiota
     • Bacterial vaginosis
       • Vaginitis
         • Candida albicans
           • Culture -  Sabouraud dextrose agar
             • In Gram stained smears, Candida appears as gram positive budding yeast cells (blastoconidia) and/or pseudohyphae
         • Trichomoniasis
         • allergic
         • STIs
           • Neisseria Gonorrhea
             • Gram Negative cocci (usually diplococci)
               • Gonococci do not contain capsules (Meningococci does).
                 • Culture: Modified Thayer Martin Medium
                   • Biochemical Tests: Oxidase Test: Positive
                     • DNase Test: Negative
           • Chlamydia Trachomatis
             • non-motile cocciod bacteria with gram negative like cell wall
               • Obligate intracellular bacteria (they were once regarded as viruses)
                 • Cytological Examination -  iodine staining of glycogen EB - Fluorescent monoclonal antibodies
                   • Serodiagnosis
                     • In Situ DNA hybridization
                       • Cultivation/Culture - cell culture  are McCoy (cycloheximide treated), Hela, Monkey kidney cells
           • Trichomas vaginalis
             • protozoan parasite
               • Microscopy and staining: wet preparation
                 • Culture - Johnson’s and Trussel’s medium
                   • Serology – NOT available
                     • Molecular Methods – PCR and NAATs
           • Treponema Pallidium
           • Treatment
             • Prevention
               • Contraceptives
                 • Hormonal
                   • Oral (the pill)
                 • Mechanical
                   • Condoms
                   • Spermicides
                   • IUD
                 • Surgical
                   • Sterilization
                   • Tubal ligation
                 • Side effects
                   • weight gain
                   • chnages in appetite and mood changes
             • Gonorrhea - Ceftriaxone
             • Chlamydia - Azithromycin
             • Syphilis - Penicillins
             • Genital herpes - acyclovir

2. Psychosocial Impact

3. causes

   • Leukorrhea
   • Midcycle
   • Intercourse
   • Atrophic vaginitis
   • Chemical irritation

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