High Performance (Pressure) Liquid Chromatography

High Performance (Pressure) Liquid Chromatography

1. Mode

   • Adsorption
   • Partition
   • Ion Exchange
   • Size Exclusion

2. Mobile Phase

   • Liquid ( pumped by high pressure)
   • Filter/ degas

3. Stationary Phase

   • Liquid ( bonded to column packing) eg. Octadecylsilane

4. Column

   • Guard Column
   • Analytical Column

5. Detector

   • UV-Visible Absorbance Detector
     • Fixed Wavelength
     • Photo-diode array
   • Mass Spectrometer Detector

6. Types of analysis

   • Isocratic Analysis
   • Gradient Elution Analysis

7. Pump

   • 500psi

8. Injector

   • Load
   • Inject