Histology

Histology

1. Light microscopy

   • Formalyn, paraffin embedding

2. TEM

   • Shows interior of the Cell
     • Glutaraldehyde and osmium tetroxide

3. SEM

   • Specimen is sputter coated with silver
     • glutaraldehyde

4. Fluorescent Microscopy

   • Immunohistochemistry
     • substrate or flourescence

5. Hematoxylin and Eosin

   • Most Common
   • Hematoxylin stains Nucleic acids & ER Blue
     • Neurons - Basophilic Nissl bodies in cytoplasm (rER)
   • Eosin stains (acidic) mitochondria Hemoglobin, Elastic Fibers red/pink

6. Periodic acid-Schiff (PAS)

   • Stain for glycoproteins Sugar
     • MicroVilli (Glycocalyx)
   • Basal lamina (Laminin) Red / Pink / Magenta
     • Type IV Collagen

7. Trichrome Blue

   • Collagen Type I

8. Silver Stain

   • Reticular Fibers
     • Type III
     • Brown / Black
   • Nerve Fibers
     • Brown / Black

9. Bright Field

   • Light beam passes through stained specimen

10. Hyaline (grape soda)

    • Type II - Basophilic
    • Aggrecan Hyaluronic Acid
    • Chondroitin Sulfate
    • A lot of smooth extra cellular matrix between cells