CELL CULTURE

CELL CULTURE

1. PREPARATION OF MEDIUM FORMS

   • CONCENTRATE
   • WORKING
   • POWERED

2. COMPOSITION AND PROPERTIES OF THE CULTURE MEDIUM

   • MINERAL SALS
   • GLUCOSE
   • VITAMINS
   • TAMPON
   • ORGANICS SUPLEMENTS, LOW MOLECULAR WEIGHT
   • HORMONES GROWTH FACTORS
     • = ANIMALS SERUM
       • + PROTEINES
   • ANTIBIOTICS ANTIFUNGALS

3. CRITERIA FOR SELECTING MEDIA

   • CELL LINES
     • THE CHOICE OF CELL CULTURE MEDIAL IS EXTREMELLY IMPORTANT & SIGNIFICANTLY AFFECTS THE SUCCES OF CELL CULTURE EXPERIMENS
   • PRIMARY CELL CULTURE
     • PRIVIDES UNIQUE & VALUABLE RESEARCH DATA
     • COMPLETE & CONDICIONED MEDIUM
       • REDUCES
         • RISK OF CONTAMINATION
         • TIME
         • LABOR
         • MONEY

4. COMMON CELL CULTURE MEDIA

   • EAGLE´S MINIUM ESSENTIAL MEDIUM (EMEM)
     • CONTAINS
       • SODIUM PYRUVATE
       • NONESSENTIAL AMINO ACIDS
       • SAL SOLUTION
     • NON-COMPLEX MEDIUM
   • RPMI-1640
     • GENERAL PURPOSE MEDIA
       • MAMMALIAN CELSS
         • HEMATOPOIETIC CELSS
         • HUMAN LYMPHOCITIES FUSION PROTOCOLS CROWTH OF HYBRID CELLS
   • DUBLECCO´S MODIFIED EAGLE´S MEDIUM (DMEM)
     • CONCENTRATIOM
       • AMINO ACIDS
       • VITAMINES
     • BASAL MEDIUM
       • WITH SUMPLEMENTATION
         • COMPLETE MEDIUM
   • DMEM/F-12
     • MIXTURE OF DMEM & HAM´S F-12
       • RICH MEDIUM
         • COMPLEX MEDIUM
       • CELLS TYPES IN BOTH SERUM
   • ISCOVE´S MODIFIED DULBECCO´S MEDIUM (IMDM)
     • CONTAINING
       • SELENIUM
       • AMINO ACIDS
       • VITAMINS
       • INORGANIC SALT
       • HEPES
       • POTASIIUM NITRATE
       • SODIUM PYRUVATE
     • GROWTH OF LYMPHOCYTES & BYBRIDOMAS
   • HAM´S NUTRIENT MIXTURES
     • F-10 HAM
       • GROWTH OF HUMAN DIPLOID CELLS & WHITE BOOD CELLS
     • F-12 HAM
       • GROWTH OF PRIMARY RAT HEPATOCITES RAT PROSTATE EPITHELIAL CELSS
     • COON F-12 HAM
       • CULTURING HYBRID CELLS
       • CONTAIN
         • DOBLE AMINO ACIDS & PIRUVATE
           • F-12 HAM
         • ASCORBIC ACID

5. INCUBATION CONDITIONS

   • Tª
     • 28Cº INSECTS 35Cº ANIMALS
   • HUMIDITY 95%

6. FEATURES PHYSIOCHEMICAL CULTURE MEDIUM

   • PH 7,2-7.6
   • OSMOLARITY 200-320 OSM/KG
   • VISCOSITY
   • SURFACE TENSION

7. GAS ATMOSPHERE

   • WERE GAS MIX CULTURES ARE MAINTAINED
   • COMPONENTS
     • OXYGEN
     • CO2