Exam

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HPLC Checklist:

HPLC definition

How are the substances separated?

Equation for partition coefficient

How is the partition coefficient determined?

-20 or 25 degrees C

-Dependent on their physical properties

What is the mobile phase?

What features are required of the mobile phase?

- Degassing: gas in solvent reduces pressure in column

- High purity

Function of a column guard

What is the stationary phase?

What features are required of the stationary phase?

-Which particle is better?

-Bonded particles are chemically and mechanically stable: longevity of column and high efficiency of chromatographic separation

- Pellicular: efficient but only accept small volumes compared to microporous

Can you draw and describe the uses of each of the HPLC components?

- Pump

- Detector

- Recorder

What are the two HPLC techniques?

How are each of the substances identified?

- Retention time

- Define retention time

-Define resolution

- Define selectivity

- Equation for selectivity

How is HPLC calibrated?

HPLC applications

HPLC advantages and disadvantages

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Cell Culture Advantages

Wide application from biochemistry to cytogenetics

There are a range of culture media recipes that are specific to the scientist's needs. These culture media can be made up by the scientist or obtained commercially, containing antifungals and antibiotics to prevent contamination

Preservation of samples for future research is easy and is carried out using liquid nitrogen and DMSO as a cryoprotectant

Population density, growth rate and culture media can be controlled

In 3D cell culture important hallmarks of tissue in vivo is able to represent a more representative model

Cell culture offers many research opportunities that are difficult to achieve in vivo

Cell Culture Disadvantages

Requires expertise is preparation of culture media, handling etc

regular routine

Subculture

days to weeks to obtain sufficient numbers

lack multicellular complexity of in vivo tissues

Aseptic technique

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