Genomes and gene technologies

Beschreibung

OCR A2 biology.
Bethany.
Quiz von Bethany., aktualisiert more than 1 year ago
Bethany.
Erstellt von Bethany. vor mehr als 10 Jahre
153
8

Zusammenfassung der Ressource

Frage 1

Frage
What is a genome?
Antworten
  • It is all of the genes possessed by an individual organisms.This includes the sequence of bases in the entire DNA of an organism.
  • It is the sequencing of an organism.
  • It is a section of the gene possessed by an organism.
  • This is all the genes possessed by all of the organisms in a similar species.

Frage 2

Frage
What are the advantages of genome sequencing?
Antworten
  • Shows evolutionary relationships.
  • Shows the effects of mutations.
  • Shows the possibility of a mutation.
  • Shows the alleles for a disease.
  • Shows the importance of bases in DNA.
  • Shows how genes can be manipulated for human benefit.
  • Helps create bio-fortified products.

Frage 3

Frage
'Micro-satellites are long sequences of DNA'.
Antworten
  • True
  • False

Frage 4

Frage
'BACs stands for Bacterial Artificial Chromosomes which are man-made plasmids'.
Antworten
  • True
  • False

Frage 5

Frage
Why are computer programmes used in genome sequencing?
Antworten
  • To compare the overlapping regions of DNA.
  • To check the criminals of the world.
  • To compare whether the genes can be bio-fortified.

Frage 6

Frage
What is genetic fingerprinting?
Antworten
  • DNA fragmentation and electrophoresis gives banding patterns unique to individuals.
  • It gives the sequence of DNA.
  • It obtains a particular gene and inserted into a recipient's organism.
  • Comparing genes for the same proteins across a range of organisms.

Frage 7

Frage
What is recombinant DNA?
Antworten
  • It is made by cutting DNA and sticking it together.
  • This occurs by conjugation of bacteria which binds two forms of plasmids.

Frage 8

Frage
'Restriction enzymes restrict the DNA from transcribing mRNA'.
Antworten
  • True
  • False

Frage 9

Frage
Why is DNA heated in automated sequencing?
Antworten
  • To break the hydrogen bonds (by denaturing them) which gives two polynucleotide chains.
  • To create a number of bases that can be amplified by PCR.
  • To separate the fragments for electrophoresis.

Frage 10

Frage
Why do the DNA fragments in electrophoresis move to the anode (positively charged electrode)?
Antworten
  • This is because they are positively charged and two positive separates them.
  • This is because they are negatively charged and the negative DNA fragments are attracted to the anode.

Frage 11

Frage
Why is buffer solution required in electrophoresis?
Antworten
  • To separate the sections of DNA.
  • To conduct electricity.

Frage 12

Frage
'The longer the fragments, the quicker they move' in electrophoresis.
Antworten
  • True
  • False

Frage 13

Frage
Primers bind at the complementary regions of DNA and probes bind at the start of DNA.
Antworten
  • True
  • False

Frage 14

Frage
Annealing is binding of base sequences.
Antworten
  • True
  • False

Frage 15

Frage
What are sticky ends?
Antworten
  • They are short, unpaired, exposed bases.
  • They are bases that are attached to restriction enzymes.

Frage 16

Frage
The insulin comes from beta cells in the liver.
Antworten
  • True
  • False

Frage 17

Frage
How is the DNA formed from the insulin gene?
Antworten
  • mRNA is extracted and incubated via reverse transcriptase which makes DNA using RNA.
  • It is completed by conjuration of bacteria formed in fermenters.
  • It is completed by the ligase enzyme.

Frage 18

Frage
Why is the ligase enzyme used to make insulin?
Antworten
  • It combines the insulin DNA and the plasmid from the bacteria (usually E. Coli).
  • Creates the backbone of DNA.

Frage 19

Frage
Golden rice is bio-fortified with vitamin B.
Antworten
  • True
  • False

Frage 20

Frage
Where is beta carotene extracted from?
Antworten
  • Daffodils
  • Bacterium: Erwina uredonma
  • Lillies
  • Wheat

Frage 21

Frage
Xenotransplantation is the transplantation of cells, tissues or organs between animals of different species.
Antworten
  • True
  • False

Frage 22

Frage
Gene therapy involves replacing a gene that are not functioning.
Antworten
  • True
  • False

Frage 23

Frage
Somatic gene therapy only affects skin cells.
Antworten
  • True
  • False

Frage 24

Frage
Genes are passed onto offspring in germ line gene therapy.
Antworten
  • True
  • False

Frage 25

Frage
What are the ethical effects of microorganisms?
Antworten
  • Cross contamination of other microorganisms.
  • Mutations of microorganisms which could lead to resistance.
  • Produce deadly toxins that lay in our food.

Frage 26

Frage
What are the temperatures of PCR that need to be set?
Antworten
  • 95, 55 and 72.
  • 75, 80 and 40.
  • 60, 40 and 81.
  • 110, 80 and 41.
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