Quiz
CELS 191 Lecture 16
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Fragen in diesem Quiz (20)
1Select the statements that apply to prokaryotic DNA
- AThy have a single, circular chromosome
- BThere is a single point of origin for DNA replication
- CDNA replication is bidirectional
- DThey have lots of linear DNA
- EThere are multiple points of origin
2Select the statements that apply to eukaryotic DNA
- AThey have lots of linear DNA
- BThere are multiple points of origin for DNA replication
- CDNA replication is bidirectional
- DThere is one, circular chromosome
- EThere is one point of origin for DNA replication
3What is the role of the primase enzyme in DNA replication
- AIt adds RNA primers to the template strand
- BIt unzips the DNA strand
- CIt releases the tension at the DNA fork
- DIt join synthesised fragments with phosphodiester bonds
4What is the role of the DNA Polymerase III enzyme in DNA replication
- AIt adds complimentary nucleotides from the reactive 3' hydroxyl group left by the RNA primer
- BIt adds complimentary base pairs from the reactive 3' phosphate group left by the RNA primer
- CIt binds at the 5' end and synthesises the new strand by adding base pairs
- DIt binds at the 3' end and synthesises the new strand by adding phosphodiester bonds
5What is the role of the Helicase enzyme in DNA replication
- AIt unwinds the DNA from the AT rich point of origin
- BIt unwinds the DNA from the point of origin
- CIt unwinds the DNA from the CG rich point of origin
- DIt puts the DNA back together after synthesis
6What is the role of the Topoisomerase enzyme in DNA replication
- AIt releases the tension at the replication fork by making cuts and then 'glues' them back together
- BIt binds to the DNA to stop the leading and lagging strand fro getting back together
- CIt unzips the gene
- DIt checks the newly added bases against the template to make sure they are correct
7What is the role of single stranded binding proteins in DNA replication
- AThey protect the single strands from degradation
- BThey prevent the single strands from coming back together again
- CThey make RNA primers
- DThe release the tension at the replication fork by making cuts and then gluing them back together again
8What is the role of Ligase in DNA replication
- AIt joins synthesised fragments with phosphodiester bonds
- BIt unzips the gene
- CIt releases the tension at the replication fork
- DIt makes RNA primers
9The overall direction of DNA synthesis on the lagging strand is in the 3'-5' direction
- ARichtig
- BFalsch
10How are errors in DNA sequence corrected during replication?
- AWith exonuclease activity of DNA Polymerase III
- BWith endonuclease activity of DNA polymerase III
- CWith exonuclease activity of DNA Polymerase II
- DWith endonuclease activity of DNA polymerase II
11[blank_start]During[blank_end] DNA replication, errors in DNA sequence are corrected with [blank_start]exonuclease[blank_end] activity of DNA polymerase [blank_start]III[blank_end]. It checks the newly added bases against the [blank_start]template[blank_end], removes it and replaces it so synthesis can continue as normal.
- Aexonuclease
- Bendonuclease
- CIII
- DII
- Etemplate
- Flagging strand
- GDuring
- HAfter
12[blank_start]After[blank_end] DNA replication, any errors in the sequence are corrected by [blank_start]endonuclease[blank_end] activity of DNA polymerase [blank_start]II[blank_end]. it removes the incorrect bases and some either side, [blank_start]DNA polymerase[blank_end] corrects the mistake, then [blank_start]Ligase[blank_end] [blank_start]joins[blank_end] the newly synthesised sequence to the pre-existing sequence with [blank_start]phosphodiester bonds[blank_end].
- AAfter
- BDuring
- Cendonuclease
- Dexonuclease
- EI
- FIII
- GDNA polymerase
- HLigase
- Ijoins
- Jdetaches
- Kphosphodiester bonds
- Lhydrogen bonds
13Failure to [blank_start]correct[blank_end] mistakes in DNA sequence will lead to [blank_start]permanent[blank_end] changes in [blank_start]DNA[blank_end] sequence, in turn leading to a [blank_start]mutation[blank_end]
- Acorrect
- Bpermanent
- CDNA
- Dmutation
14PCR (P[blank_start]olymerase[blank_end] C[blank_start]hain[blank_end] R[blank_start]eaction[blank_end]) [blank_start]amplifies[blank_end] a gene so you can have [blank_start]multiple[blank_end] copies of the [blank_start]same[blank_end] gene. There are no [blank_start]lagging[blank_end] strands (unlike prokaryotic replication) as both strands are synthesised [blank_start]continuously[blank_end].
- Aolymerase
- Bhain
- Ceaction
- Damplifies
- Emultiple
- Fsame
- Glagging
- Hcontinuously
15Select the key components of PCR
- ADNA template
- BPrimers
- CHeat stable DNA polymerase
- DdNTPS
- EBuffer solution
- FDivalent Cations
- GCovalent Anions
- HRNA template
- IddNTPS
- JDNA polymerase
16At what temperature does Denaturation of double stranded DNA occur during PCR?
- A95C
- B45-70C
- C72C
- D85C
17At what temperature does Annealing of DNA templates occur during PCR?
- A95C
- B45-70C
- C72C
- D105C
18At what temperature does extension/elongation occur during PCR?
- A72C
- B95C
- C50C
- D40-75C
19What is the role of DNA polymerase I during DNA replication
- AIt removes the RNA primers on Okazaki fragments
- BIt fills in the gaps left by removed RNA primers with complimentary nucleotides
- CIt adds the RNA primers on Okazaki fragments
- DIt fills in the gaps left by removed DNA primers with RNA
20DNA replication begins with [blank_start]Helicase[blank_end] unwinding the DNA strands from an [blank_start]AT[blank_end] rich origin of replication, with [blank_start]Single[blank_end] [blank_start]Stranded[blank_end] [blank_start]Binding[blank_end] [blank_start]Proteins[blank_end] (SSBP) protecting the strands from [blank_start]degradation[blank_end] and stopping them from coming back together again. Topoisomerase releases [blank_start]tension[blank_end] at the replication fork by cutting and gluing the strands back together again. [blank_start]Primase[blank_end] adds an [blank_start]RNA[blank_end] primer with a chemically reactive [blank_start]3[blank_end]’ hydroxyl group, then DNA Polymerase [blank_start]III[blank_end] adds complimentary [blank_start]nucleotides[blank_end] from that hydroxyl group. On the leading strand, synthesis happens [blank_start]continuously[blank_end] in the [blank_start]5'-3'[blank_end] direction, however on the lagging strand this occurs [blank_start]discontinuously[blank_end] in the overall [blank_start]3'-5'[blank_end] direction in [blank_start]Okazaki[blank_end] fragments. RNA primers are added intermittently along the strand, with DNA Polymerase III adding the complementary nucleotides in the 5’-3’ direction. DNA Polymerase [blank_start]I[blank_end] then comes and removes the RNA primers on the leading and lagging strands, and fills in the gaps with [blank_start]complimentary[blank_end] nucleotides. [blank_start]Ligase[blank_end] forms the bonds between 3’ hydroxyl and 5’ phosphate groups, completing DNA synthesis.
- AHelicase
- BAT
- CSingle
- DStranded
- EBinding
- FProteins
- Gdegradation
- Htension
- IPrimase
- JRNA
- K3
- LIII
- Mnucleotides
- Ncontinuously
- O5'-3'
- Pdiscontinuously
- Q3'-5'
- ROkazaki
- SI
- Tcomplimentary
- ULigase
Informationen
- Beschreibung
- Medicine (CELS 191) Quiz am CELS 191 Lecture 16, erstellt von Eva Bonning am 24/05/2017.
- Autor
- Eva Bonning
- Sprache
- English
- Aufrufe
- 29
- Schlagwörter
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