Test
CELS 191 Lecture 16
Este test tiene tipos de pregunta que se abren en el reproductor de siempre.
Preguntas del test (20)
1Select the statements that apply to prokaryotic DNA
- AThy have a single, circular chromosome
- BThere is a single point of origin for DNA replication
- CDNA replication is bidirectional
- DThey have lots of linear DNA
- EThere are multiple points of origin
2Select the statements that apply to eukaryotic DNA
- AThey have lots of linear DNA
- BThere are multiple points of origin for DNA replication
- CDNA replication is bidirectional
- DThere is one, circular chromosome
- EThere is one point of origin for DNA replication
3What is the role of the primase enzyme in DNA replication
- AIt adds RNA primers to the template strand
- BIt unzips the DNA strand
- CIt releases the tension at the DNA fork
- DIt join synthesised fragments with phosphodiester bonds
4What is the role of the DNA Polymerase III enzyme in DNA replication
- AIt adds complimentary nucleotides from the reactive 3' hydroxyl group left by the RNA primer
- BIt adds complimentary base pairs from the reactive 3' phosphate group left by the RNA primer
- CIt binds at the 5' end and synthesises the new strand by adding base pairs
- DIt binds at the 3' end and synthesises the new strand by adding phosphodiester bonds
5What is the role of the Helicase enzyme in DNA replication
- AIt unwinds the DNA from the AT rich point of origin
- BIt unwinds the DNA from the point of origin
- CIt unwinds the DNA from the CG rich point of origin
- DIt puts the DNA back together after synthesis
6What is the role of the Topoisomerase enzyme in DNA replication
- AIt releases the tension at the replication fork by making cuts and then 'glues' them back together
- BIt binds to the DNA to stop the leading and lagging strand fro getting back together
- CIt unzips the gene
- DIt checks the newly added bases against the template to make sure they are correct
7What is the role of single stranded binding proteins in DNA replication
- AThey protect the single strands from degradation
- BThey prevent the single strands from coming back together again
- CThey make RNA primers
- DThe release the tension at the replication fork by making cuts and then gluing them back together again
8What is the role of Ligase in DNA replication
- AIt joins synthesised fragments with phosphodiester bonds
- BIt unzips the gene
- CIt releases the tension at the replication fork
- DIt makes RNA primers
9The overall direction of DNA synthesis on the lagging strand is in the 3'-5' direction
- AVerdadero
- BFalso
10How are errors in DNA sequence corrected during replication?
- AWith exonuclease activity of DNA Polymerase III
- BWith endonuclease activity of DNA polymerase III
- CWith exonuclease activity of DNA Polymerase II
- DWith endonuclease activity of DNA polymerase II
11[blank_start]During[blank_end] DNA replication, errors in DNA sequence are corrected with [blank_start]exonuclease[blank_end] activity of DNA polymerase [blank_start]III[blank_end]. It checks the newly added bases against the [blank_start]template[blank_end], removes it and replaces it so synthesis can continue as normal.
- Aexonuclease
- Bendonuclease
- CIII
- DII
- Etemplate
- Flagging strand
- GDuring
- HAfter
12[blank_start]After[blank_end] DNA replication, any errors in the sequence are corrected by [blank_start]endonuclease[blank_end] activity of DNA polymerase [blank_start]II[blank_end]. it removes the incorrect bases and some either side, [blank_start]DNA polymerase[blank_end] corrects the mistake, then [blank_start]Ligase[blank_end] [blank_start]joins[blank_end] the newly synthesised sequence to the pre-existing sequence with [blank_start]phosphodiester bonds[blank_end].
- AAfter
- BDuring
- Cendonuclease
- Dexonuclease
- EI
- FIII
- GDNA polymerase
- HLigase
- Ijoins
- Jdetaches
- Kphosphodiester bonds
- Lhydrogen bonds
13Failure to [blank_start]correct[blank_end] mistakes in DNA sequence will lead to [blank_start]permanent[blank_end] changes in [blank_start]DNA[blank_end] sequence, in turn leading to a [blank_start]mutation[blank_end]
- Acorrect
- Bpermanent
- CDNA
- Dmutation
14PCR (P[blank_start]olymerase[blank_end] C[blank_start]hain[blank_end] R[blank_start]eaction[blank_end]) [blank_start]amplifies[blank_end] a gene so you can have [blank_start]multiple[blank_end] copies of the [blank_start]same[blank_end] gene. There are no [blank_start]lagging[blank_end] strands (unlike prokaryotic replication) as both strands are synthesised [blank_start]continuously[blank_end].
- Aolymerase
- Bhain
- Ceaction
- Damplifies
- Emultiple
- Fsame
- Glagging
- Hcontinuously
15Select the key components of PCR
- ADNA template
- BPrimers
- CHeat stable DNA polymerase
- DdNTPS
- EBuffer solution
- FDivalent Cations
- GCovalent Anions
- HRNA template
- IddNTPS
- JDNA polymerase
16At what temperature does Denaturation of double stranded DNA occur during PCR?
- A95C
- B45-70C
- C72C
- D85C
17At what temperature does Annealing of DNA templates occur during PCR?
- A95C
- B45-70C
- C72C
- D105C
18At what temperature does extension/elongation occur during PCR?
- A72C
- B95C
- C50C
- D40-75C
19What is the role of DNA polymerase I during DNA replication
- AIt removes the RNA primers on Okazaki fragments
- BIt fills in the gaps left by removed RNA primers with complimentary nucleotides
- CIt adds the RNA primers on Okazaki fragments
- DIt fills in the gaps left by removed DNA primers with RNA
20DNA replication begins with [blank_start]Helicase[blank_end] unwinding the DNA strands from an [blank_start]AT[blank_end] rich origin of replication, with [blank_start]Single[blank_end] [blank_start]Stranded[blank_end] [blank_start]Binding[blank_end] [blank_start]Proteins[blank_end] (SSBP) protecting the strands from [blank_start]degradation[blank_end] and stopping them from coming back together again. Topoisomerase releases [blank_start]tension[blank_end] at the replication fork by cutting and gluing the strands back together again. [blank_start]Primase[blank_end] adds an [blank_start]RNA[blank_end] primer with a chemically reactive [blank_start]3[blank_end]’ hydroxyl group, then DNA Polymerase [blank_start]III[blank_end] adds complimentary [blank_start]nucleotides[blank_end] from that hydroxyl group. On the leading strand, synthesis happens [blank_start]continuously[blank_end] in the [blank_start]5'-3'[blank_end] direction, however on the lagging strand this occurs [blank_start]discontinuously[blank_end] in the overall [blank_start]3'-5'[blank_end] direction in [blank_start]Okazaki[blank_end] fragments. RNA primers are added intermittently along the strand, with DNA Polymerase III adding the complementary nucleotides in the 5’-3’ direction. DNA Polymerase [blank_start]I[blank_end] then comes and removes the RNA primers on the leading and lagging strands, and fills in the gaps with [blank_start]complimentary[blank_end] nucleotides. [blank_start]Ligase[blank_end] forms the bonds between 3’ hydroxyl and 5’ phosphate groups, completing DNA synthesis.
- AHelicase
- BAT
- CSingle
- DStranded
- EBinding
- FProteins
- Gdegradation
- Htension
- IPrimase
- JRNA
- K3
- LIII
- Mnucleotides
- Ncontinuously
- O5'-3'
- Pdiscontinuously
- Q3'-5'
- ROkazaki
- SI
- Tcomplimentary
- ULigase
Información
- Descripción
- (CELS 191) Medicine Test sobre CELS 191 Lecture 16, creado por Eva Bonning el 24/05/2017.
- Autor
- Eva Bonning
- Idioma
- English
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