Transposons

TRANSPOSONS

A transposon is a genetic element that can be translocated as a whole segment, between chromosomal, phage and plasmid DNA, in the absence of a complementary sequence in the host. Transposons are ubiquitous. Transposons induce a large fraction of spontaneous mutations and chromosomal rearrangements. Sometimes, transposons can encode antibiotic resistance, and so can confer this between species. 

Insertion sequences 

7d1152db-fd20-4e70-8a11-ad794431f00b (image/png)

Pie de foto: : Insertion sequence schematic

The most simple form of transposon. This sequence encodes only the protein needed for its own transposition - transposase. In E. coli, IS1 is present 4-19 times depending on the strain. The ends of all known IS elements show inverted terminal repeats, or ITRs of 20-50 base pairs. Note that the more widespread a transposon is throughout organisms, the lower the insertional bias it will exhibit. 

Composite transposons

236d31db-2551-4925-8709-f6405ba4f478 (image/png)

A composite transposon consists of a central gene (e.g. antibiotic resistance), flanked by two identical IS elements. The IS elements provide transposase and ITR recognition elements (normally only one IS element transcriptionally active).

Pie de foto: : Composite transposon schematic

560a762a-986f-4dd3-ad17-b89f4588d7a6 (image/png)

Non composite transposons

Pie de foto: : Non composite transposon schematic

These are much like the composite transposons, however they do not contain flanking IS elements, however they are flanked by ITRs. These transposons encode genes from their own transposition and accessory function (AB resistance). Transposition requires 2 enzymes:  Transposase (like composite transposons)  Resolvase 

Conjugative transposons 

c9267e66-8f5b-4268-8a10-b38136924826 (image/png)

Pie de foto: : Schematic of conjugative transposon mode of action

Sometimes called an ICE (integrated conjugative element), the conjugative transposon will excise its flanks and recombines them outside of the chromosome as a circular DNA. Mobility functions encoded on the plasmid enable it to conjugate itself from one cell to another, using rolling circle replication mechanism - however remember it can not replicate itself, so it must integrate back into the host chromosome to be replicated. 

Mechanisms of transposition 

Conservative/non replicative: A 'cut and paste' mechanism. The transposase creates dsDNA cuts in the donor, as well as staggered cuts in the recipient. This is non replicative, therefore donor DNA is lost or repaired. The product on the recipient strand is a scar (direct repeat of target DNA) either side of the transposon - this is a good indicator of transposon presence. IS and composite transposons. Replicative:A 'copy and paste' mechanism. The transposase makes two ssDNA cuts (one each strand) in the donor and staggered cuts in the target. A cointegrate is formed and resolvase is needed to resolve the structure. The product is again, a direct repeat either side of the transposon. Non composite transposons. 

5ed8521d-c73f-46dd-945a-4023cf1d60b7 (image/png)

Pie de foto: : Cointegrate schematic

DNA rearrangements products

Transposons are major contributors to genetic variation on the chromosome. This happens by:  Insertions - by transposition or homologous recombination (cut and paste introduces repeat scars which are mutations) Deletions - homologous recombination between 2 copies of a transposon, present in direct orientation  Inventions - homologous recombination between 2 copies of a transposon, present in inverted orientation 

e91b15d3-2d66-4886-af92-01c149be64c1 (image/png)