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HT 12 Flotation Bath Troubleshooting

Vilmida Castro Inglés 12 fichas

HT 12 Flotation Bath Troubleshooting

Fichas del conjunto (12)

  1. 1Cross contaminationThe water surface is always skimmed between specimens to avoid contamination of one section with cells from another.
    Care is taken not to brush hair or hands whilst floating-out sections (squames can contaminate sections).
  2. 2debrissquamesdebris
  3. 3cross contamination /debristissue contamination
    ex: thyroid tissue in cardiac section
    can lead to misdiagnosis
    cross contamination /debris
  4. 4foldsfolds
  5. 5wrinklewrinkle
  6. 6wavy sectionnot flatwavy section
  7. 7parched earthwater bath too hot
    or not optimal processing
    parched earth
  8. 8air bubblesSmall air bubbles in the flotation bath. Any bubbles that are trapped under the section apparently disappear as the section dries. Although the bubbles may apparently disappear, the areas in the section above the bubble are often distorted and are likely to float off during staining.air bubbles
  9. 9incomplete deparaffinationincomplete deparaffination
  10. 10retraction/nuclear bubblingattributed to presence of water in nuclei freshly cut and immediately dry (above 60C)retraction/nuclear bubbling
  11. 11retraction/shrinkageretraction/shrinkage
  12. 12Adhesive artifactex excessive albuminAdhesive artifact

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Fichas sobre HT 12 Flotation Bath Troubleshooting, creado por Vilmida Castro el 10/06/2019.
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Vilmida Castro
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