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Jenny Lai Inglés 93 fichas

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  1. 1What type of test is this?oxidation-fermentation testWhat type of test is this?
  2. 2what color are gram positive stains?purple
  3. 3what color are gram negative stains?red
  4. 4What is the oxidation fermentation t test used for?differentiate bacteria on the basis of fermentative or oxidative metabolism of carbohydrates
  5. 5what is the mineral oil in O-F test used for?creates an environment unsuitable for oxidation
  6. 6O-F test results: sealed (anaerobic) green or blueoxidationO-F test results: sealed (anaerobic) green or blue
  7. 7O-F test results: sealed and unsealed yellow throughoutoxidation and fermentation, or fermentation onlyO-F test results: sealed and unsealed yellow throughout
  8. 8O-F test results: sealed or unsealed slightly yellow at the topslow oxidation and slow fermentation, or slow fermentation only
  9. 9O-F test results: sealed or unsealed no color change or blueno sugar metabolismO-F test results: sealed or unsealed no color change or blue
  10. 10O-F test results: unsealed yellowoxidize onlyO-F test results: unsealed yellow
  11. 11What type of test is this?Phenol red testWhat type of test is this?
  12. 12what is a phenol red test used for?determine organism's ability to ferment that particular carbohydrate
    *single carbohydrate used
    glucose, lactose, and sucrose
  13. 13Phenol Red test result: yellow with bubblefermentation with acid and gasPhenol Red test result: yellow with bubble
  14. 14Phenol Red test result: yellow no bubblefermentation with acid no gasPhenol Red test result: yellow no bubble
  15. 15Phenol Red test result: red no bubbleno fermentationPhenol Red test result: red no bubble
  16. 16Phenol Red test result: pink no bubbledegradation of peptonePhenol Red test result: pink no bubble
  17. 17what type of test is this?catalase testwhat type of test is this?
  18. 18what is a Catalase test used for?determine organisms that contain catalase to use or live in the presence of 02
  19. 19Catalase test result: bubblecatalase is present +Catalase test result: bubble
  20. 20Catalase test result: no bubblescatalase absent -Catalase test result: no bubbles
  21. 21what type of test is this?oxidase testwhat type of test is this?
  22. 22what is an oxidase test used for?identify organisms that produce the enzyme cytochrome oxidase
  23. 23Oxidase test result: dark blue/purplecytochrome C oxidase is present +Oxidase test result: dark blue/purple
  24. 24Oxidase test result: no color changecytochrome C oxidase is absent -Oxidase test result: no color change
  25. 25what type of test is this?MacConkey Testwhat type of test is this?
  26. 26what is a MacConkey test used for?determine ability to ferment lactose in gram-negative bacteria
  27. 27why are gram + unable to grow in MacConkey test agar?inhibited by bile salts and crystal violet
  28. 28MacConkey test result: pink/redproduces acid from fermentation +MacConkey test result: pink/red
  29. 29MacConkey test result: colorlessdoes not ferment -MacConkey test result: colorless
  30. 30what type of test is this?gelatinase testwhat type of test is this?
  31. 31what is a gelatinase test used for?identify enzymes that can hydrolyze gelatin with gelatinases
  32. 32Gelatinase test result: gelatin is liquidgelatinase is present +Gelatinase test result: gelatin is liquid
  33. 33Gelatinase test result: gelatin is solidgelatinase is absent -Gelatinase test result: gelatin is solid
  34. 34what type of test is this?starch hydrolysis testwhat type of test is this?
  35. 35what is a starch hydrolysis test used for?determine if an organism can hydrolyse starch
    *iodine will color area surrounding the growth if growth is present
  36. 36Starch hydrolysis test: clearing around growthamylase is present +Starch hydrolysis test: clearing around growth
  37. 37Starch hydolysis test: no clearingamylase is absent -Starch hydolysis test: no clearing
  38. 38what type of test is this?citrate utilization testwhat type of test is this?
  39. 39what is the citrate utilization test used for?differentiate faculatative anaerobes, determine those with the ability to ferment carbohydrates, use citrate as sole source
    ferment carb=functional citric acid cycle=can aerobically respire
  40. 40citrate utilization test result: blue or no color change w/ growthcitrate is utilized +citrate utilization test result: blue or no color change w/ growth
  41. 41citrate utilization test: no color change, no growthcitrate is not utilized -citrate utilization test: no color change, no growth
  42. 42what type of test is this?nitrate reduction testwhat type of test is this?
  43. 43what is a nitrate reduction test used for?determine whether an organism can reduce nitrate to nitrite or free nitrogen gas
  44. 44nitrate reduction test: what step is needed next?none
    positive, nonfermenter    nitrate reduction test: what step is needed next?
  45. 45nitrate reduction test: what is done next to a fermenter?add reagent A & B, if it turns red + stop therenitrate reduction test: what is done next to a fermenter?
  46. 46nitrate reduction test: what do you do if there is no color change after the reagents are added?add zinc
  47. 47nitrate reduction test: what does the result indicate if zinc has been added?nitrate reduction, +nitrate reduction test: what does the result indicate if zinc has been added?
  48. 48nitrate reduction test: what does the result indicate if zinc has been added?no nitrate reduction -nitrate reduction test: what does the result indicate if zinc has been added?
  49. 49what type of test is this?decarboxylase testwhat type of test is this?
  50. 50what is a decarboxylase test used for?determine if organism can decarboxylate an amino acid
    *add mineral oil
  51. 51decarboxylase test result: no color changeno decarboxylation -decarboxylase test result: no color change
  52. 52decarboxylase test: yellowfermentation, no decarboxylation -decarboxylase test: yellow
  53. 53decarboxylation test: purpledecarboxylation +decarboxylation test: purple
  54. 54what type of test is this?blood agar testwhat type of test is this?
  55. 55what is a blood agar test used for?differentiate bacteria based on their ability to hemolyze RBCs
  56. 56blood agar test result: clearing around growthhemolyzes RBC completely βblood agar test result: clearing around growth
  57. 57blood agar test result: greening around growthpartially hemolyzes αblood agar test result: greening around growth
  58. 58blood agar test result: no changedoes not hemolyze γblood agar test result: no change
  59. 59what type of test is this?MR testwhat type of test is this?
  60. 60what is the purpose of the MR test?detect organisms capable of performing a mixed acid fermentation to overcome the phosphate buffer
    *add methyl red indicator (3 drops) immediate results
  61. 61MR test result: redmixed acid fermentation +MR test result: red
  62. 62MR test result: no color changeno mixed acid fermentation -MR test result: no color change
  63. 63what type of test is this?VP testwhat type of test is this?
  64. 64what is a VP test used for?identify organisms that can ferment glucose
    *add 15 drops of reagent A and 5 drops of reagent B
  65. 65VP test result: redfermentation, acetoin produced +VP test result: red
  66. 66VP test result: no color changeno fermentation -VP test result: no color change
  67. 67what type of test is this?deamination testwhat type of test is this?
  68. 68what is the purpose of a phenylalanine deaminase test?identify organism that can produce phenylalanine deaminase to deaminate amino acid phenylalanine
    *add ferric chloride
  69. 69phenylalanine deaminase test result: greendeaminase present +phenylalanine deaminase test result: green
  70. 70phenylalanine deaminase test result: no color changeno deaminase -phenylalanine deaminase test result: no color change
  71. 71what type of test is this?SIM testwhat type of test is this?
  72. 72what is the purpose of the SIM test?determine sulfur reduction, indole production from tryptophan, and motility
  73. 73Sulfur reduction test result: blacksulfur reduction +Sulfur reduction test result: black
  74. 74Sulfur reduction test result: no blackno sulfur reduction -Sulfur reduction test result: no black
  75. 75what needs to be added for indole production?Kovac's reagent, reacts with indole
  76. 76Indole production result: redpresence of tryptophanase +Indole production result: red
  77. 77Indole production result: no redno tryptophanase -Indole production result: no red
  78. 78Motility result: growth radiating/fuzzymotility +Motility result: growth radiating/fuzzy
  79. 79Motility result: no radiating growthno motility -Motility result: no radiating growth
  80. 80what type of test is this?Mannitol salt agarwhat type of test is this?
  81. 81what is the purpose of the mannitol salt agar test?differentiates Staphylococcus aureus from other Staphylococcus species
  82. 82Mannitol salt agar results: poor growth/ no growthinhibited by NaCl, not Staphylococcus
  83. 83Mannitol salt agar results: good growthnot inhibited, Staphylococcus
  84. 84Mannitol salt agar results: yellow growth or halomannitol fermentation, Staphylococcus aureusMannitol salt agar results: yellow growth or halo
  85. 85Mannitol salt agar results: red growthnot fermentation, other type of StaphylococcusMannitol salt agar results: red growth
  86. 86what type of test is this?Kligler Iron Agarwhat type of test is this?
  87. 87what is the purpose of the Kliger Iron Agar?differentiate bacteria on the basis of sulfur reduction and glucose, lactose, and sucrose fermentation
  88. 88KIA test result: yellow slant/ yellow buttglucose and lactose fermentation with acid accumulationKIA test result: yellow slant/ yellow butt
  89. 89KIA test result: red slant, yellow buttglucose and lactose and/or sucrose fermentation with acid accumulationKIA test result: red slant, yellow butt
  90. 90KIA test result: red slant, red buttno fermentation, peptone catabolized aerobic and anaerobicKIA test result: red slant, red butt
  91. 91KIA test result: red slant, no change in buttno fermentation, peptone aerobicKIA test result: red slant, no change in butt
  92. 92KIA test result: black precipitatesulfur reductionKIA test result: black precipitate
  93. 93KIA test result: crackgas productionKIA test result: crack

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Fichas sobre notes, creado por Jenny Lai el 11/05/2018.
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Jenny Lai
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