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Lab Practicum 2: Exercise 7
Fichas del conjunto (10)
- 1smearthin layer of microbes spread over a clean microscopic slide and then allowed to bone dry (air dry)
- 2simple stainstaining procedure using only one day.
stains cells directly
determines cell, size, shape, and arrangement
positively charged colored ion (basic dye) - 3direct stainsimple stain
- 4negative stainstains the background. negatively charged colored ion (anionic)
- 5pleomorphismirregularity in form.
demonstrates several different cell shapes - 6what information is provided by a simple stainsize, shape, and arrangement
- 7purpose of heat-fixing smear(i) kill bacteria
(ii) adhere it to slide - 8what problems can arise as a result of heat-fixingchange in shape, distortion of cell morphology, break slide
- 9regarding heat-fixing problem, what technique solves issue?negative staining
does not involve heat, results in no cell distortion - 10why is thickness of a smear important?distinguish. cant see size/shape/arrangement if clumped together
using a stain is for coloring, specimen wont be stained/rinsed properly if smear is to thick (uneven staining)
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- Fichas sobre Lab Practicum 2: Exercise 7, creado por newsha el 22/10/2014.
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- newsha
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